摘要
      抗微生物耐药性（AMR）是一个全球性的健康问题。如今，抗生素抗性基因（ARGs）被认为是新出现的污染物。这项研究遵循了“一个健康”框架，在该框架中，包括野生动物在内的环境中的AMR监测对于缓解这一问题是可取的。在这里，我们调查了与欧亚水獭相关的AMR，欧亚水獭是一种半水生哺乳动物，被认为是淡水健康的指标。为此，通过高通量qPCR阵列对水獭的粪便耐药性进行了表征。该技术具有高容量的ARG剖面。此外，我们还评估了从水獭扭伤中分离的两种指示细菌，大肠杆菌和肠球菌的抗菌敏感性，并根据临床和流行病学截断值（ECOFF）对结果进行了解释。
Abstract
Antimicrobial resistance (AMR) is a global health concern. Nowadays, antibiotic resistance genes (ARGs) are considered emerging pollutants. This study followed the One Health framework, in which AMR surveillance in the environment, including in wild animals, is advisable to mitigate this problem. Here we investigated AMR associated with Eurasian otter, a semi-aquatic mammal considered an indicator of freshwater health. To do so, otter's faecal resistome was characterized by a high-throughput qPCR array. This technique has a high-capacity of ARGs profiling. Additionally, we have assessed the antimicrobial susceptibility of two indicator bacteria, E. coli and Enterococcus spp, isolated from otter spraints and interpreted the results according to clinical and epidemiological cut-offs (ECOFFs).

https://www.sciencedirect.com/science/article/pii/S004565352203137X