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微藻-细菌联合体处理猪废水的研究:共生机制和抗性基因分析

发布者:抗性基因网 时间:2023-06-07 浏览量:234

摘要
      本研究考察了微藻-细菌联合体(MBC)(蛋白核小球藻活性污泥(AS))处理低C/N比猪废水的效果。共培养后,NH4+-N和PO43-P的去除率分别提高了53.84%和43.52%。此外,MBC对磺胺甲恶唑(SMX)的降解率略高于活性污泥法。有趣的是,MBC出水中抗生素抗性基因(ARGs)的绝对丰度相对低于AS工艺。蛋白核梭菌具有负ζ电位,使细菌能够粘附在其表面。与AS过程相比,MBC细胞外聚合物(EPS)中碳水化合物和蛋白质的浓度显著增加。在门水平上,变形菌门、拟杆菌门和蓝细菌门是MBC的主要细菌,而子囊菌门和担子菌门是主要真菌。总的来说,这些发现有助于更好地了解MBC处理含有抗生素的猪废水。
Abstract
This study investigated the effects of microalgae-bacteria consortia (MBC) (Chlorella pyrenoidosa-activated sludge (AS)) treating swine wastewater with low C/N ratios. After co-culture, the removal rates of NH4+-N and PO43--P increased by 53.84% and 43.52%. Furthermore, the sulfamethoxazole (SMX) degradation rates in MBC were slightly higher than in the activated sludge process. Interestingly, the absolute abundance of antibiotic resistance genes (ARGs) in effluent from MBC is relatively less than in the AS process. C. pyrenoidosa has a negative zeta potential that allows bacteria to adhere to its surface. The concentrations of carbohydrates and proteins in extracellular polymeric substance (EPS) of MBC dramatically increased compared with the AS process. At the phylum level, Proteobacteria, Bacteroidota, and Cyanobacteria were the main bacteria, while Ascomycota and Basidiomycota were the primary fungi in MBC. Overall, those findings lead to a better understanding of the swine wastewater containing antibiotic treatment by MBC.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422002218