摘要
      抗微生物耐药性（AMR）是全球最重要的健康威胁之一。准确识别耐药菌株和个体抗微生物耐药性基因（ARGs）的能力对于理解AMR的进化和出现以及提供适当的治疗至关重要。下一代测序技术的快速发展使这项技术可供世界各地常规实验室的研究人员和微生物学家使用。然而，对于那些在生物信息学方面经验有限的人来说，缺乏可用的工具，特别是使中低收入国家的研究人员和微生物学家能够进行自己的研究。CGE工具（基因组流行病学中心），包括ResFinder(https://cge.cbs.dtu.dk/services/ResFinder/)开发的目的是提供免费提供的易于使用的在线生物信息学工具，使缺乏经验的研究人员和微生物学家能够进行简单的生物信息学分析。主要目的过去和现在都是为参与一线诊断的人员，特别是LMIC的人员提供这些解决方案。自2012年首次发表以来，ResFinder经历了一系列改进，包括改进代码和数据库，包括选定细菌物种的点突变，以及对选定物种表型的预测。截至2021年9月28日，使用ResFinder对171个国家的61776个IP地址进行了820803次分析。ResFinder显然满足了全球几个人的需求，我们希望未来能够继续免费提供这项服务。我们还希望并期望提供进一步的改进，包括对其他细菌物种的表型预测。
Abstract
Antimicrobial resistance (AMR) is one of the most important health threats globally. The ability to accurately identify resistant bacterial isolates and the individual antimicrobial resistance genes (ARGs) is essential for understanding the evolution and emergence of AMR and to provide appropriate treatment. The rapid developments in next-generation sequencing technologies have made this technology available to researchers and microbiologists at routine laboratories around the world. However, tools available for those with limited experience with bioinformatics are lacking, especially to enable researchers and microbiologists in low- and middle-income countries (LMICs) to perform their own studies. The CGE-tools (Center for Genomic Epidemiology) including ResFinder (https://cge.cbs.dtu.dk/services/ResFinder/) was developed to provide freely available easy to use online bioinformatic tools allowing inexperienced researchers and microbiologists to perform simple bioinformatic analyses. The main purpose was and is to provide these solutions for people involved in frontline diagnosis especially in LMICs. Since its original publication in 2012, ResFinder has undergone a number of improvements including improvement of the code and databases, inclusion of point mutations for selected bacterial species and predictions of phenotypes also for selected species. As of 28 September 2021, 820 803 analyses have been performed using ResFinder from 61 776 IP-addresses in 171 countries. ResFinder clearly fulfills a need for several people around the globe and we hope to be able to continue to provide this service free of charge in the future. We also hope and expect to provide further improvements including phenotypic predictions for additional bacterial species.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914360/