摘要

包含四环素（TC）的粪肥对土壤中抗生素抗性基因的流行的影响仍然知之甚少。在此，三种不同的TC（土霉素（OTC），四环素（TC）和金霉素（CTC））分别与未污染的粪肥混合以施肥土壤。土壤接受140天内每14天单次施用0μg/ kg，300μg/ kg（TC和CTC）或700μg/ kg（OTC）或多次施用。监测四种四环素抗性基因（TRG），包括tet（A），tet（L），tet（M）和tet（Q）。尽管单次施用处理中四种TRG的丰度起初迅速增加，但随着时间的推移它们逐渐减少，并且显着低于112天后的重复处理。尽管所有添加的TC在第140天停止，但我们继续评估长TRGs的长期积累。大部分TRGs甚至在TC含有粪肥未超过15个月后才被检测到。在含有TC的肥料停止施肥后，TRG的丰度在重复施用处理中高于单次施用处理。因此，应更多地关注含有抗生素的粪肥在耕地中的应用。

The influence of manure containing tetracyclines (TCs) on the prevalence of antibiotic resistance genes in soils remains poorly understood. Here, three different TCs (oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC)) were mixed respectively with unpolluted manure to fertilize arable soil. The soil received either a single application of 0 μg kg−1, 300 μg kg−1 (TC and CTC), or 700 μg kg−1 (OTC) or multiple applications every 14 days for 140 days. Four tetracycline resistance genes (TRGs), including tet(A), tet(L), tet(M), and tet(Q), were monitored. Although the abundances of the four TRGs in the single application treatment initially increased rapidly, they decreased over time and were significantly lower than those of the repeated treatments after day 112. All additions of TCs stopped on day 140, but we continued to assess the long-term accumulation of TRGs. Most of the TRGs were detected even after the TC-containing manures had not been applied for more than 15 months. The abundance of the TRGs after ceasing fertilization with the TC-containing manures was higher in the repeated application treatments than in the single application treatments. Therefore, more attention should be paid to repeated applications of antibiotic-containing manure to arable soils.

https://link.springer.com/article/10.1007/s11356-017-0853-x