摘要

厌氧氨氧化物社区在高效废水处理中的重要作用日益受到关注。然而，细菌交流方面的细菌相互作用仍然是难以捉摸的。本研究首先利用生物反应器操作过程中获得的宏基因组序列数据，探讨厌氧氨氧化菌群内细菌的种内和种间交流。我们通过与包含11种鉴定的基因亚型的构建的BCG数据库进行比对来验证多种细菌通讯基因（BCG）亚型的存在。初始启动时细菌通讯比高负荷阶段更活跃，并且与细菌多样性逐渐减少相关。 Hdts（产生种内信号分子AHL的关键基因之一）和RpfF（产生种内和种间信号传导分子DSF的关键基因）是厌氧氨氧化菌群中的主要通讯引擎，因为它们的丰度很高。 Anammox细菌主要使用Hdts基因与其他基因进行交流，而RpfF基因起着核心作用，其特征在于与其他BCG亚型的多重相关性。有趣的是，具有丰富BCG的细菌更倾向于与具有相同功能性状的细菌相互作用，这表明除了经常报道的底物共同利用之外，这些细菌之间潜在的与通讯相关的相互作用。这突出显示了AHL和DSF对厌氧氨氧化菌群的主要重要性，从而暗示了目标调节信号的潜在策略，以提高厌氧氨氧化活性和废水处理的竞争能力。

Increasing attention has been paid to the anammox community for its significant function in high-efficiency wastewater treatment. However, bacterial interaction in terms of bacterial communication is still elusive. This study firstly explored the intra- and interspecific communication of bacteria in the anammox community using metagenomicsequence data obtained during bioreactor operation. We verified the existence of multiple bacterial communication gene (BCG) subtypes by alignment with the constructed BCG database containing 11 identified gene subtypes. Bacterial communication was more active at the initial start-up than in the high loading-rate phase, and was correlated with the gradually decreasing bacterial diversity. Hdts, one of the key genes that produced the intraspecific signaling molecule AHL, and RpfF, the key gene that produced the intra- and interspecific signaling molecule DSF, were the primary communication engines in the anammox community because of their high abundance. Anammox bacteria mainly used Hdts genes to communicate with others, while RpfF gene played a core role characterized by their multiple correlations with other BCG subtypes. Interestingly, bacteria with abundant BCGs were more inclined to interact with the bacteria with the same functional traits, indicating the potential communication-related interaction among these bacteria in addition to the frequently reported substrate co-utilization. This highlights the primary importance of AHL and DSF for the anammox community, and thereby hints at a potential strategy for the target regulation of the signals to improve anammox viability and competitive capacity in wastewater treatment.

https://www.sciencedirect.com/science/article/pii/S0043135418301611