摘要
      研究了猪粪厌氧发酵过程中不同初始pH值（即3、5、7、11）对细胞内和细胞外抗生素抗性基因（iARGs和eARGs）以及携带ARG的潜在微生物宿主的影响。在pH 3和pH 5下，几乎所有iARGs和eARGs的丰度都下降了0.1–1.7个对数。在pH 7和pH 11下，只有三种iARG和eARG的丰度下降了0.1–0.9 log。在酸性初始发酵条件下（pH 3和pH 5），ARG的去除效果更明显。酸性条件（pH 3和pH 5）显著降低了微生物群落的多样性和丰度，从而消除了许多潜在的ARG宿主和抗生素耐药性致病菌（ARPB）。因此，研究结果有助于研究猪粪厌氧发酵对ARGs和ARPB去除和污染风险的影响。
Abstract
Effects of various initial pH values (i.e., 3, 5, 7, 11) during anaerobic fermentation of swine manure on intracellular and extracellular antibiotic resistance genes (iARGs and eARGs) and ARG-carrying potential microbial hosts were investigated. The abundance of almost all iARGs and eARGs decreased by 0.1–1.7 logs at pH 3 and pH 5. The abundance of only three iARGs and eARGs decreased by 0.1–0.9 logs at pH 7 and pH 11. Under acidic initial fermentation conditions (pH 3 and pH 5), the ARG removal effect was more pronounced. Acidic conditions (pH 3 and pH 5) significantly reduced the diversity and abundance of the microbial community, thereby eliminating many potential ARG hosts and antibiotic-resistant pathogenic bacteria (ARPB). Therefore, the study results contribute to the investigation of the effects of swine manure anaerobic fermentation on the removal and risk of contamination of ARGs and ARPB.

https://www.sciencedirect.com/science/article/abs/pii/S0960852423001323