摘要
      该研究旨在使用不依赖于培养的基于废水的流行病学监测（WBE）方法来评估城市环境的社区范围内的抗微生物耐药性（AMR）状况。在连接污水处理厂（STP）的排水管的汇合点收集生活污水样本。评估收集的水样中是否存在125个抗生素抗性基因（ARGs）和13个可移动遗传元件（MGE、5个整合子和8个转座子）。还检查了抗生素残留和细菌群落的组成。社区污水表现出不同的耐药性模式，靶向ARGs的阳性检测尤其丰富，尤其是aph、aadA1和strB。对氨基糖苷类和甲氧苄啶类的耐药性普遍存在，其次是氯霉素、磺酰胺和β-内酰胺类。根据微生物多样性评估，变形菌门、拟杆菌门、厚壁菌门和绿弯菌门是观察到的丰富的门，而螺旋菌科、假单胞菌科和莫拉克菌科是常见的科。该研究提供了社区范围内ARG的全面基线信息，将用于ARG的预防和管理。
Abstract
The study aimed to evaluate the community-wide antimicrobial resistance (AMR) profile of an urban setting using the culture-independent wastewater-based epidemiological surveillance (WBE) approach. The domestic wastewater sample was collected at the converging point of the drain connecting the Sewage Treatment Plant (STP). The collected water sample was evaluated for the presence of 125 antibiotic resistance genes (ARGs) and 13 mobile genetic elements (MGEs, 5 integrons and 8 transposons). Antibiotic residues and the composition of bacterial communities were also examined. Community's sewage showed a diverse resistance pattern, with the positive detection of targeted ARGs, notably aph, aadA1, and strB being particularly abundant. Resistance to aminoglycoside and trimethoprim classes was prevalent, followed by chloramphenicol, sulfonamide, and β-lactams. According to the microbial diversity assessment, Proteobacteria, Bacteroidetes, Firmicutes, and Chloroflexi were abundant phyla observed, while Helicobacteraceae, Pseudomonadaceae, and Moraxellaceae were prevalent families. The study provided comprehensive baseline information of ARGs on a community scale and will be of use for ARG prevention and management.

https://www.sciencedirect.com/science/article/abs/pii/S0048969723000347