摘要

由于猪用于饲料促进生长和控制疾病，高浓度的残留阿散酸会出现在猪粪中。 本研究比较了三种浓度（0,325和650 mg / kg干猪粪）的阿散酸对厌氧消化过程中抗生素抗性基因（ARG）和微生物群落丰度的影响。 添加650mg / kg阿散酸使消化产物中tetC，sul2，ermB和gyrA的绝对丰度提高超过两倍。 冗余分析表明，微生物群落结构的变化是ARGs变化的主要驱动因素。 As抗性基因arsC与四种ARG和intI1共同发生，可能导致阿糖酸在压力下ARGs的增加。 高阿斯利酸浓度可增加厌氧消化产物中发生ARG的风险。 应该控制用作饲料添加剂的阿散酸的量。

High concentrations of residual arsanilic acid occur in pig manure due to its use in feed to promote growth and control diseases. This study compared the effects of arsanilic acid at three concentrations (0, 325, and 650 mg/kg dry pig manure) on the abundance of antibiotic resistance genes (ARGs) and the microbial community during anaerobic digestion. Addition of 650 mg/kg arsanilic acid enhanced the absolute abundances oftetC, sul2, ermB, and gyrA more than twofold in the digestion product. Redundancy analysis indicated that the change in the microbial community structure was the main driver of variation in the ARGs profile. The As resistance gene arsC co-occurred with four ARGs and intI1, possibly causing the increase in ARGs under pressure by arsanilic acid. High arsanilic acid concentrations can increase the risk of ARGs occurring in anaerobic digestion products. The amount of arsanilic acid used as a feed additive should be controlled.

https://www.sciencedirect.com/science/article/pii/S096085241730295X