摘要

土壤中的抗生素和抗生素抗性基因（ARG）可以通过食物链影响人体健康。生物炭是一种土壤改良剂，但其对ARGs和与土壤和蔬菜相关的微生物群落的影响尚不清楚。因此，我们建立了三个生菜盆培养实验，即O300：300mg / kg土霉素（OTC），BO300：300mg / kg OTC + 2％生物炭，以及没有OTC或生物炭的对照。我们发现，在BO300条件下，与O300相比，莴苣叶，根和土壤中ARG的相对丰度分别降低了51.8％，43.4％和44.1％。 intI1在土壤和莴苣中含量很高，与一些ARG（tetW，ermF和sul1）共同发生。冗余分析和网络分析表明，细菌群落演替是影响ARGs和intI1变异的主要机制。由生物炭处理土壤和莴苣引起的厚壁菌减少是造成叶中四环素抗性基因去除的主要因素。生物炭的应用导致人类致病菌（HPB）的消失，这与ermF和ermX的丰度显着相关。总之，生物炭是一个有效的农田修正案，用于减少抗生素，ARGs和HPB的含量，以确保蔬菜安全和保护人类健康。

Antibiotics and antibiotic resistance genes (ARGs) in soil can affect human health via the food chain. Biochar is a soil amendment but its impacts on ARGs and the microbial communities associated with soil and vegetables are unclear. Therefore, we established three lettuce pot culture experiments, i.e., O300: 300 mg/kg oxytetracycline (OTC), BO300: 300 mg/kg OTC + 2% biochar, and a control without OTC or biochar. We found that under BO300, the relative abundances of ARGs were reduced by 51.8%, 43.4%, and 44.1% in lettuce leaves, roots, and soil, respectively, compared with O300. intI1 was highly abundant in soil and lettuce, and it co-occurred with some ARGs (tetW, ermF, andsul1). Redundancy analysis and network analysis indicated that the bacterial community succession was the main mechanism that affected the variations in ARGs and intI1. The reduction of Firmicutes due to the biochar treatment of soil and lettuce was the main factor responsible for the removal of tetracycline resistance genes in leaves. Biochar application led to the disappearance of human pathogenic bacteria (HPB), which was significantly correlated with the abundances of ermF and ermX. In summary, biochar is an effective farmland amendment for reducing the abundances of antibiotics, ARGs, and HPB in order to ensure the safety of vegetables and protect human health.

https://www.sciencedirect.com/science/article/pii/S026974911630793X