摘要

      如今，一个难题是通过处理和未处理的水扰乱人们的健康，即在这些环境中存在抗生素耐药性细菌。第一个是这些生物通过遗传因素获得抗性基因的能力，第二个似乎是这些物质的潜在毒性。供水中缺乏大肠杆菌、大肠杆菌、铜绿假单胞菌和肠肠球菌等细菌的健康指数。这项研究的主要目的是弄清楚这些细菌是如何作为抗生素耐药性库发挥作用的，不仅要找到SHV、TEM和CTX-M等耐药性基因，还要描绘德黑兰供水样本中克隆的树状图。在一年的时间里，对17株铜绿假单胞菌进行了分析。在这项研究中，94.1%的分离物的TEM基因检测呈阳性，而只有一个分离物的CTX-M基因检测呈阴性，没有一个分离株的SHV基因检测呈呈阳性。这项研究表明，铜绿假单胞菌在饮用水中并不像以前认为的那样常见。然而，铜绿假单胞菌似乎可以垂直传播获得性抗生素耐药性，而不是水平传播。Nowadays, a difficult problem has been disrupting people's health through treated and untreated water, namely the existence of antibiotic-resistant bacteria in these environments. The first is such organisms’ ability to acquire resistance genes through genetic elements, and the second seems to be the substances’ potential toxicity. The health index of supply water is lacking for bacteria such as Coliform bacteria, Escherichia coli, Pseudomonas aeruginosa, and Intestinal Enterococci. The study's main aim was to figure out how these bacteria function as antibiotic resistance pools, not only to find resistant genes like SHV, TEM, and CTX-M but also to depict the dendrogram of clones in water supply samples in Tehran. Over the course of a year, 17 strains of P. aeruginosa were analyzed. In this study, 94.1% of the isolates tested positive for the TEM gene, while only one isolate tested positive for the CTX-M gene, and none tested positive for the SHV gene. This research shows that P. aeruginosa is not as common in drinkable water as previously thought. Nevertheless, it appears that P. aeruginosa can spread acquired antibiotic resistance vertically rather than horizontally.

https://iwaponline.com/ws/article/doi/10.2166/ws.2023.088/94503/Detection-of-antibiotic-resistant-bacteria-and