摘要
      苄基二甲基铵化合物（BACs）通常用作表面活性剂和消毒剂。在本研究中，硝化系统分别暴露于不同的烷基链长度（C12-C16）和不同水平的BACs（0-5mg/L），总共120天，以探索BACs对抗性基因（RGs）的慢性影响。RGs根据活性污泥的性质分为四个部分。BACs对氨氧化性能没有显著影响，而BACs增加了污泥中大多数细胞内RGs的绝对丰度。在BACs的暴露下，水中的细胞外RGs（we RGs）呈下降趋势，si RGs倾向于转化为we RGs。紧密结合的酪氨酸侧链与大多数we RGs显著相关，并且we-intI1可能有助于RGs的传播。因此，应认真对待在BACs胁迫下硝化系统中不同组分RGs传播的风险。
Abstract
Benzalkyl dimethylammonium compounds (BACs) are generally applied as surfactants and disinfectants. In this study, the nitrification systems were exposed to different alkyl chain lengths (C12-C16) and different levels of BACs (0-5 mg/L), respectively, totally 120 days and to explore the chronic effect of BACs on resistance genes (RGs). RGs were classified into four fractions based on activated sludge properties. Ammonia oxidation performance were not significantly affected by BACs, whereas BACs increased the absolute abundance of most intracellular RGs in sludge (si-RGs). Under the exposure of BACs, extracellular RGs in water (we-RGs) showed a decrease trend and si-RGs tended to be converted to we-RGs. Tightly bound-Tyrosine side chain was significantly correlated with most we-RGs, and we-intI1 might contribute to the propagation of RGs. Therefore, the risk of transmission of different fractions of RGs in the nitrification system under the stress of BACs should be taken seriously.

https://www.sciencedirect.com/science/article/abs/pii/S0960852423000147