摘要
      抗微生物耐药性（AMR）已成为世界范围内最大的挑战之一，阻碍了大量感染的治疗。事实上，AMR危机对实现联合国可持续发展目标构成了威胁，由于其多部门性质，需要采取全面的方法来解决这一问题。因此，对诊所以外的环境进行调查是至关重要的。在这里，我们调查了从巴西城市河口分离的13株抗微生物气单胞菌。大多数菌株携带至少一个抗微生物耐药性基因，11株携带至少一种重金属耐药性基因。值得注意的是，四株（30.7%）菌株携带blaKPC基因，编码碳青霉烯酶。特别是，确定了嗜水气单胞菌34SFC-3菌株的全基因组序列，不仅揭示了抗菌和重金属抗性基因的存在，而且揭示了一个多功能的病毒库。还检测到了可移动的遗传元件，包括插入序列、转座子、整合偶联元件和IncQ1质粒。考虑到气单胞菌的普遍性、它们的遗传混杂性、致病性和承受环境压力的内在特征，我们的研究结果强化了嗜水气单胞菌确实是“各行各业的杰克”这一概念，从“一个健康”的角度来看，这一点不应被忽视。
Abstract
Antimicrobial resistance (AMR) has become one of the greatest challenges worldwide, hampering the treatment of a plethora of infections. Indeed, the AMR crisis poses a threat to the achievement of the United Nations’ Sustainable Development Goals and, due to its multisectoral character, a holistic approach is needed to tackle this issue. Thus, the investigation of environments beyond the clinic is of utmost importance. Here, we investigated thirteen strains of antimicrobial-resistant Aeromonas isolated from an urban estuary in Brazil. Most strains carried at least one antimicrobial resistance gene and 11 carried at least one heavy metal resistance gene. Noteworthy, four (30.7%) strains carried the blaKPC gene, coding for a carbapenemase. In particular, the whole-genome sequence of Aeromonas hydrophila strain 34SFC-3 was determined, revealing not only the presence of antimicrobial and heavy metal resistance genes but also a versatile virulome repertoire. Mobile genetic elements, including insertion sequences, transposons, integrative conjugative elements, and an IncQ1 plasmid were also detected. Considering the ubiquity of Aeromonas species, their genetic promiscuity, pathogenicity, and intrinsic features to endure environmental stress, our findings reinforce the concept that A. hydrophila truly is a “Jack of all trades’’ that should not be overlooked under the One Health perspective.

https://www.mdpi.com/2079-6382/12/1/84