摘要
      对氯间甲苯酚（PCMC）是一种多用途防腐剂，广泛应用于各种医药个人护理产品中。在本研究中，对活性污泥进行PCMC原位厌氧浸泡，以启动、维持和恢复部分硝化（PN）。最佳处理条件为80 mg/L PCMC和30 h暴露时间，可稳定维持PN长达157天。结果表明，PCMC对胞外聚合物的含量和二级结构有很大影响，从而增强了细菌对PCMC的聚集和耐受性。分子对接模拟表明PCMC通过抑制亚硝酸盐氧化还原酶实现了长期PN。微生物群落结果表明，PCMC对亚硝酸盐氧化菌有较强的抑制作用，氨氧化菌在数量和寡聚型上都富集，可以适应PCMC。同时，PCMC可以明显阻断一些抗性基因的富集，而不会增加系统的抗性基因传递负担。PCMC是一种新型且有前途的PN抑制剂。
Abstract
The p-chloro-m-cresol (PCMC) is a versatile preservative with extensive appliance in various pharmaceutical personal care products. In this study, PCMC in-situ anaerobic soaking of activated sludge was implemented to initiate, maintain and restore partial nitrification (PN). The optimal treatment condition was 80 mg/L PCMC and 30 h exposure time, which stably sustained PN for up to 157 days. Results showed that PCMC had a great influence on the content and secondary structures of extracellular polymeric substances, thus enhanced the aggregation and tolerance of bacteria to PCMC. The molecular docking simulation illustrated that PCMC achieved long-term PN by inhibiting nitrite oxidoreductase. The microbial community results showed that PCMC had a stronger inhibitory effect on nitrite oxidizing bacteria, and ammonia oxidizing bacteria was enriched in both quantity and oligotypes which could adapt to PCMC. Meanwhile, PCMC can obviously block the enrichment of some resistance genes without increasing resistance gene transmission burden of system. PCMC is a novel and promising PN inhibitor.

https://www.sciencedirect.com/science/article/abs/pii/S1385894723004424