摘要

碳青霉烯抗性肠杆菌科不仅仅是从人类环境中分离出来的，而且也是从家畜中分离出来的。我们首次报道了德国一家畜牧场VIM-1碳青霉烯酶的存在。在这些农场发现的VIM-1抗性基因位于IncHI2质粒上。为了能够更详细地分析这些质粒，来自单个农场的两种不同质粒（来自沙门氏菌enterica的pRH-R27和来自大肠杆菌的pRH-R178）被完全测序并分析抗生素和重金属抗性的存在。质粒显示含有blaVIM-1，aacA4，aadA1，sul1，qacEΔ（编码在In110 1类整合子中）以及blaACC-1，strA / strB和catA1基因以及重金属抗性（ter-， mer-，sil-，ars-，rcn-和pco）。与其他IncHI2质粒比较表明，尽管pRH-R27是一种嵌合型IncHI2质粒，与质粒pSTm-A54650和R478（均是从人类分离的）都具有高度同源性，但是pRH-R178是pRH-R27的缺失衍生物，推测可能是由几个IS介导的缺失表明质粒在该环境中的遗传进化。

Carbapenem-resistant Enterobacteriaceae are not any more isolated only from human settings, but also from livestock. We reported for the first time the presence of VIM-1 carbapenemases in a livestock farm in Germany. The VIM-1 resistance gene found in these farms was located on IncHI2 plasmids. In order to be able to analyse these plasmids in more detail, two different plasmids from a single farm (pRH-R27 from Salmonella enterica and pRH-R178 from Escherichia coli) were completely sequenced and analysed for the presence of antibiotic and heavy metal resistances. The plasmids showed to harbour blaVIM-1, aacA4,aadA1, sul1, qacEΔ (encoded in an In110 class 1 integron), as well as blaACC-1, strA/strB, and catA1 genes together with resistance to heavy metals (ter-, mer-, sil-, ars-, rcn-, andpco). Comparison with other IncHI2 plasmid revealed that while pRH-R27 is a mosaic IncHI2 plasmid with both high homology to the plasmid pSTm-A54650 and R478, both isolated from humans, pRH-R178 is a deletion derivative of pRH-R27, presumably caused by several IS-mediated deletions indicating genetic evolution of plasmids in this environment.

https://www.sciencedirect.com/science/article/pii/S0378113515300201