摘要

链霉菌属H-KF8是一种从智利巴塔哥尼亚峡湾海洋沉积物中分离出来的放线菌菌株。在各种培养基中评估了形态特征以及抗菌活性，揭示了主要针对革兰氏阳性菌（金黄色葡萄球菌和单核细胞增生李斯特菌）的碳源依赖性活性。该抗菌细菌的基因组挖掘揭示了26种生物合成基因簇（BGC）用于次级代谢产物，其中81％与已知BGC具有低相似性。此外，链霉菌中的基因组搜索。 H-KF8揭示了与重金属抗性（49个基因），氧化应激（69个基因）和抗生素抗性（97个基因）相关的各种遗传决定因子的存在。这项研究揭示了海洋来源的链霉菌属。 H-KF8细菌能够耐受多种重金属，如铜，钴，汞，铬酸盐和镍;以及高毒性亚碲酸盐，这是首次描述链霉菌的特征。另外，Streptomyces sp。与土壤参考菌紫色链霉菌A3（2）相比，H-KF8具有对氧化应激的主要抗性。此外，链霉菌（Streptomyces sp。） H-KF8对所测试的抗生素显示出88％的抗性，表明总体上对几种非生物胁迫物有强烈的反应。这些生物性状的组合证实了链霉菌的代谢多功能性。 H-KF8是一种基因良好的微生物，能够对抗峡湾独特的海洋环境。

Streptomyces sp. H-KF8 is an actinobacterial strain isolated from marine sediments of a Chilean Patagonian fjord. Morphological characterization together with antibacterial activity was assessed in various culture media, revealing a carbon-source dependent activity mainly against Gram-positive bacteria (S. aureus and L. monocytogenes). Genome mining of this antibacterial-producing bacterium revealed the presence of 26 biosynthetic gene clusters (BGCs) for secondary metabolites, where among them, 81% have low similarities with known BGCs. In addition, a genomic search inStreptomyces sp. H-KF8 unveiled the presence of a wide variety of genetic determinants related to heavy metal resistance (49 genes), oxidative stress (69 genes) and antibiotic resistance (97 genes). This study revealed that the marine-derived Streptomyces sp. H-KF8 bacterium has the capability to tolerate a diverse set of heavy metals such as copper, cobalt, mercury, chromate and nickel; as well as the highly toxic tellurite, a feature first time described for Streptomyces. In addition, Streptomyces sp. H-KF8 possesses a major resistance towards oxidative stress, in comparison to the soil reference strain Streptomyces violaceoruber A3(2). Moreover, Streptomycessp. H-KF8 showed resistance to 88% of the antibiotics tested, indicating overall, a strong response to several abiotic stressors. The combination of these biological traits confirms the metabolic versatility of Streptomyces sp. H-KF8, a genetically well-prepared microorganism with the ability to confront the dynamics of the fjord-unique marine environment.

https://peerj.com/articles/2912/?utm_source=TrendMD&utm_campaign=PeerJ_TrendMD_1&utm_medium=TrendMD