摘要

在这项工作中，我们评估了太阳光的潜力以及用太阳光辅助的光芬顿过程来灭活废水中的抗生素抗性细菌（ARB）（WW）。对基于太阳能的过程之后ARB灭活和再生动力学的系统研究以及它们的抗生素抗性（AR）的修饰。因此，作为模型ARB的链霉素抗性（SR）大肠杆菌在各种辐照水平下经受日光暴露（有或没有UVB光），以及在中性pH下的太阳能/ H 2 O 2和光 - 芬顿过程。我们报道了SR大肠杆菌对已知动力学模型（> 96％R2）的良好拟合，并且分析性地给出了完全灭活所需的治疗时间，并且停止了它们的治疗后再生长能力。对于所有的治疗方法，发现AR在治疗期间减少，在可培养性丧失之前（4-log失活时间减少5-15％）。 UVB辐射被确定为失去栽培性，AR和再生的关键因素。太阳能/ H2O2和photo-Fenton呈现快速灭活率，确保ARB不再生，并且表明对修饰大肠杆菌SR具有中等效果，两种菌株的灭活时间相似。发现WW中链霉素的存在对所有测试过程中的更快失活起协同作用。最后，发现约1小时的太阳能AOPs可确保4-log ARB灭活，即使在没有残留H2O2的情况下也不会再生，表明它们适合作为适当的WW消毒过程。

In this work, we assess the potential of solar light and the solar-assisted photo-Fenton process to inactivate antibiotic resistant bacteria (ARB) in wastewater (WW). A systematic investigation on ARB inactivation and regrowth kinetics after solar-based processes is intended, as well as the modification of their antibiotic resistance (AR). As such, a Streptomycin-resistant (SR) E. coli as a model ARB was subjected to solar exposure (with or without UVB light) at various irradiance levels, as well as the solar/H2O2 and the photo-Fenton process at neutral pH. We report the good fit of the SR E.coli to known kinetic models (>96% R2), and analytically present the necessary treatment timed for total inactivation and halting their post-treatment regrowth capability. For all treatment methods, the AR was found to decrease during treatment, prior to loss of cultivability (5–15% lower time for 4-log inactivation). UVB irradiation was determined as the key factor of loss of cultivability, AR and regrowth. Solar/H2O2 and photo-Fenton presented fast inactivation rates, ensured no regrowth of ARB, and indicated moderate effect on modifying the SR of E. coli, with similar inactivation times for both strains. The presence of Streptomycin in WW was found to act synergistically on the faster inactivation by all processes tested. Finally, ∼1 h of solar-based AOPs was found to ensure 4-log ARB inactivation and no regrowth, even in absence of the residual H2O2, indicating their suitability as proper WW disinfection processes.

https://www.sciencedirect.com/science/article/pii/S0920586117307307