摘要

全球范围内正在发生抗生素失败。在对天然水体抗生素抗性基因（ARGs）的常规监测研究中，我们注意到检测出了粘菌素抗性基因mcr-1，这种基因先前在几个国家的人和动物的大肠杆菌和肺炎克雷伯菌分离株中发现。 mcr-1基因可能存在于水环境中，因为水生生态系统被认为是抗生素抗性细菌（ARB）和ARGs的储库。在这项研究中，开发了qPCR分析来监测和量化中国海河的mcr-1基因。结果表明，沿海河流域6个月以上不同地点采集的18份样本均为mcr-1基因阳性，mcr-1最高水平达到每升水3.81×105个基因拷贝（GC）。这是首次通过qPCR定量天然水体系中mcr-1的研究。我们的研究结果强调了这种抗生素耐药决定因素的广泛传播潜力，表明其对健康和生态的重大影响。

Antibiotic failure is occurring worldwide. In a routine surveillance study on antibiotic resistance genes (ARGs) in natural water bodies, we noted the detection of colistin-resistance gene mcr-1, previously identified in Escherichia coliand Klebsiella pneumoniae isolates from human beings and animals in several countries. The mcr-1 gene might be present in water environments, because aquatic ecosystems are recognized as reservoirs for antibiotic resistant bacteria (ARB) and ARGs. In this study, a qPCR assay was developed to monitor and quantify the mcr-1 gene in the Haihe River, China. The results showed that all 18 samples collected from different locations over 6 months along the Haihe River were positive for the mcr-1 gene, and the highest level of mcr-1 reached 3.81 × 105gene copies (GC) per liter of water. This is the first study to quantify mcr-1 in a natural water system by qPCR. Our findings highlight the potential for this antibiotic resistance determinant to spread extensively, suggesting a significant health and ecological impact.

http://www.mdpi.com/1660-4601/14/6/576/htm