摘要

羊奶分离株sakei GM3具有耐酸、耐胆汁性，对食源性致病菌具有拮抗活性。sakei GM3菌株产生的细菌素18小时后在固定相达到最大产量，经不同pH、温度和有机溶剂处理后，细菌素仍保持活性，但对某些蛋白水解酶表现出敏感性。经盐析、尺寸排阻色谱、C18柱反相高效液相色谱和MALDI - TOF - MS体系检测，分子量为4.8 kDa。细菌素GM3对HT29细胞系的细胞毒性在2240 AU / ml浓度下表现出最大的存活抑制( 45.60±0.5 % )。结果表明，L. sakei GM3具有作为生物防腐剂应用于食品工业的潜力。

Goat milk isolate Lactobacillus sakei GM3 exhibited acid and bile tolerance and showed antagonistic activity against food borne pathogens. Bacteriocin produced by L. sakei GM3 reached maximum production at stationary phase after 18 h. Bacteriocin was found to be retained active after treatment with different pH, temperature and organic solvents but showed sensitivity to some proteolytic enzymes. The molecular mass was found to be 4.8 kDa after subjecting to salting out, size exclusion chromatography, reversed-phase HPLC on C18 column and MALDI-TOF-MS system. The cytotoxicity on HT29 cell line by bacteriocin GM3 showed maximum survival inhibition (45.60 ± 0.5%) at 2240 AU/ml concentration. The results suggest that the L. sakei GM3 has the potential to be used in food industry as a biopreservative.

https://www.sciencedirect.com/science/article/pii/S0956713516302055#!