摘要

乳酸菌产生的细菌素是核糖体合成的抗菌肽，具有不同的杀菌活性模式。本研究着重于细菌素ST8SH的杀菌活性的表征，特别关注控制李斯特菌和肠球菌物种。植物乳杆菌ST8SH产生的抗菌肽PA-1家族的细菌素（在遗传水平上具有96%的相似性），对几种实验室、肠球菌、肺炎克雷伯菌、李斯特菌、Streptococcus spp.和其他一些人类和食源性病原体具有活性。单核细胞增生李斯特氏菌和粪肠球菌ATCC 19433的指数或固定相培养物中添加细菌素ST8SH抑制生长12 h，通过酶、蛋白质和核苷酸物质渗漏间接记录ST8SH对单核细胞增生李斯特菌和粪肠球菌ATCC 19433的影响。考虑到细菌素ST8SH对受试微生物的抗菌活性，以及植物乳杆菌ST8SH的生理特性，细菌素或菌株可作为生物保存的工具。

Bacteriocins produced by lactic acid bacteria (LAB) are ribosomally synthesized antimicrobial peptides, with a diverse mode of bactericidal activity. This study focused on characterization of the bactericidal activity of bacteriocin ST8SH, with special attention to control of Listeria andEnteroccus species. Lactobacillus plantarum ST8SH produces a bacteriocin of the pediocin PA-1 family (sharing 96 % similarity on genetic level) with activity against several LAB,Enterococcus spp., Klebsiella pneumoniae, Listeria spp., Streptococcus spp. and some other human and foodborne pathogens. Addition of bacteriocin ST8SH to exponential or stationary phase cultures of L. monocytogenes ScottA and E. faecalis ATCC 19433 inhibited growth for 12 h. The effects of bacteriocin ST8SH on L. monocytogenes ScottA and E. faecalis ATCC 19433 were recorded indirectly based on enzyme, protein and nucleotide material leakage. Considering the antimicrobial activity of bacteriocin ST8SH against the tested microorganisms, and the physiological characteristics of Lb. plantarum ST8SH, either the bacteriocin or the strain may be used as tools for biopreservation.

https://link.springer.com/article/10.1007/s13213-015-1180-4