摘要

生物膜在食品工业中广泛存在，并且与几种食物相关疾病的爆发有关。生物膜也是他们对抗微生物剂耐药性的一个原因。在本研究中，使用两种生物活性化合物，即从海洋细菌获得的黑色素和细菌素，实现生物膜形成食物病原体的生物防治。观察到部分纯化的黑色素和细菌素BL8显示极大减少了食物病原体的生物膜形成，即使是微量的，也显示出高的抗生物膜活性。多重抗生素抗性（MAR）指数显示九种食物病原体的多重抗性。研究中使用的黑色素的FTIR光谱显示出两个峰，这是标准黑色素IR光谱的特征。扫描电子显微照片显示在用两种生物活性化合物处理之前和之后微生物质量和生物膜形成的变化，显然显示它们的抗生物膜活性。

Biofilms are widespread and a bane in food based industry for being associated with the outbreaks of several food related diseases. Biofilms are also a cause for concern for their resistance to antimicrobial agents. In the present study, the biocontrol of biofilm forming food pathogens was achieved using two bioactive compounds, namely, melanin and bacteriocin, obtained from marine bacteria. Partially purified melanin and bacteriocin BL8 were observed to show great reduction in the biofilm formation of food pathogens, even in minute quantities, and showed high antibiofilm activity. Multiple antibiotic resistance (MAR) index showed the multiple resistance of nine food pathogens. FTIR spectrum of the melanin used in the study showed two peaks, which are the characteristic features of standard melanin IR spectrum. Scanning electron micrographs showed the variation in the microbial mass and biofilm formation before and after treatment with the two bioactive compounds, evidently showing their antibiofilm activity.

http://nopr.niscair.res.in/handle/123456789/39311