摘要

农杆菌108是抗坏血酸 - 碳环环戊烯酮类似物的3'-O-β-D-吡喃木糖基 - 胞苷-5'-氧 - 磷酸二酯，具有细菌素样特性。 该细菌素比氨苄青霉素或链霉素的指示菌株的抑菌圈直径大几个数量级。 它已经从发根土壤杆菌菌株K108的培养物中分离出来。 农作物108的结构没有详细说明，之前已经公布。 我们现在报告了详细的结构阐明，包括迄今未确定的残留5'-磷酸二酯片段，通过在H 2 O / D 2 O中的各种pH值下的1D和2D NMR研究，高分辨率MS，pKa测定和化学降解的组合进行研究。

Agrocin 108 is a 3′-O-β-D-xylopyranosyl-cytidine-5′-O-phosphodiester of an ascorbate-carbocyclic cyclopentenone analogue, with bacteriocin-like properties. This bacteriocin exhibits orders of magnitude greater than the inhibition zone diameter towards the indicator strain than either ampicillin or streptomycin. It has been isolated from cultures of Rhizobium rhizogenes strain K108. The structure of the agrocin 108 without detail, has been previously published. We now report a detailed structure elucidation, including the hitherto undetermined residual 5′-phospho-diester fragment by a combination of 1D and 2D NMR studies at various pH values in H2O/D2O, high resolution MS, pKa determination, and chemical degradation.

https://www.nature.com/articles/s41429-017-0014-y