摘要

这项研究旨在开发一种抗微生物可生物降解的食品包装，用于控制世界上消耗量最大的淡水鱼之一pangasius鱼片中的病原体。将细菌素7293（Bac7293）（一种来自Weissella hellenica BCC 7293的新型细菌素）扩散涂布到聚（乳酸）/锯末颗粒生物复合膜（PLA / SP）上，最大量为19.54±2.87μg/ cm 2。根据JIS Z 2801：2000标准测试方法，所制备的浸渍有Bac7293（PLA / SP + Bac7293）的PLA / SP膜有效抑制革兰氏阳性（单核细胞增多性李斯特菌和金黄色葡萄球菌）和革兰氏阴性菌（铜绿假单胞菌，嗜水气单胞菌，大肠杆菌和鼠伤寒沙门氏菌），这已被认为是拒绝pangasius鱼片的原因。与对照相比，这种抗微生物膜通过挑战试验的方式抑制目标微生物的生长约2-5log CFU / cm 2，与对照相比，体外和pangasius鱼片均依赖于细菌菌株。 PLA / SP + Bac7293薄膜在食品模拟物中的总体迁移在异辛烷中最大（3.67±0.47 mg / dm2），远低于欧盟委员会规定的整体迁移限值。这表明PLA / SP + Bac7293作为pangasius鱼片的良好抗菌包装的高潜力应用。

This study aimed to develop an antimicrobial biodegradable food packaging for control of pathogens in pangasius fish fillets, one of the world's most consumed freshwater fish.Bacteriocin 7293 (Bac7293), a novel bacteriocin from Weissella hellenica BCC 7293, was diffusion coated onto poly (lactic acid)/sawdust particle biocomposite film (PLA/SP) with the maximum amount of 19.54 ± 2.87 μg/cm2. According to the JIS Z 2801:2000 standard testing method, the produced PLA/SP film impregnated with Bac7293 (PLA/SP + Bac7293) effectively inhibited both Gram-positive (Listeria monocytogenes and Staphylococcus aureus) and Gram-negative bacteria (Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli and Salmonella Typhimurium) which have been considered as a reason for the rejection of pangasius fish fillets. This antimicrobial film inhibited the growth of target microorganisms by about 2–5 log CFU/cm2, depended on bacterial strains, compared with control, both in vitro and in pangasius fish fillets by means of challenge test. The overall migration of PLA/SP + Bac7293 film to food simulants was maximized in isooctane (3.67 ± 0.47 mg/dm2) which was much lower than the overall migration limit regulated by the commission of the European communities. This suggested high potential application of PLA/SP + Bac7293 as a good antimicrobial packaging for pangasius fish fillets.

https://www.sciencedirect.com/science/article/pii/S0023643817307648