摘要

细菌素是由细菌产生的蛋白质毒素以抑制类似或密切相关的细菌的生长。在乳酸菌（LAB）中，细菌素由链球菌，片球菌，乳酸杆菌等产生。近年来，产细菌素的LAB由于其通常被认为是安全状态和潜在用作食品保存的安全添加剂而引起了关注。细菌素在包装薄膜中的应用，以控制食物腐败和病原生物，这是近十年来积极研究的一个领域。抗菌包装膜通过包装与食品表面的直接接触来防止食品表面上的微生物生长。这项研究的目标是分离细菌素生产实验室从Yakult®，开发抗菌包装系统，并评估其对选定的腐败和病原微生物的抗菌作用。因此，通过使用干酪乳杆菌的细菌素并将其涂布或吸附到不同包装材料的表面上来制造抗菌包装膜。通过针对测试生物体大肠杆菌和金黄色葡萄球菌的琼脂扩散测定来测试涂覆膜的抗微生物活性。所获得的结果证明，细菌素可用于抑制两种测试生物体。因此，可以使用细菌素开发抗菌包装系统，从而降低病原体发展的风险，并延长食品的保质期。

Bacteriocins are proteinaceous toxin produced by bacteria to inhibit the growth of similar or closely related bacteria. Among lactic acid bacteria (LAB), bacteriocins are produced by Streptococcus, Pediococcus, Lactobacillus, etc. In recent years, bacteriocin-producing LAB have attracted significant attention because of their generally recognized as safe status and potential use as safe additives for food preservation. Incorporation of bacteriocins into packaging films to control food spoilage and pathogenic organisms has been an area of active research for last decade. Antimicrobial packaging film prevents microbial growth on food surface by direct contact of the package with the surface of food. The objectives of this study were to isolate bacteriocin-producing LAB from Yakult®, develop antimicrobial packaging system and evaluate their antimicrobial effects on selected spoilage and pathogenic microorganisms. For this reason, the antimicrobial packaging film was made by using the bacteriocin by Lactobacillus casei and coating it or adsorbing it onto the surface of different packaging materials. The antimicrobial activity of the coated films was tested by agar diffusion assay against the test organisms Escherichia coli and Staphylococcus aureus. The results obtained proved that bacteriocins can be used to inhibit both the test organisms. Thus antimicrobial packaging systems can be developed using bacteriocins thereby reducing the risk of pathogen development, as well as extending the shelf life of foods.

https://www.pagepress.org/journals/index.php/mr/article/view/6622