乳酸链球菌肽是在发酵过程中由乳酸乳球菌产生的含有34个氨基酸的多环肽。最近，研究人员认为乳酸链球菌肽是一种抗癌肽。在此，作者旨在评估乳酸链球菌肽对结肠癌细胞系中细胞凋亡刺激的作用。将SW480细胞暴露于不同浓度的乳链菌肽，细胞活力以及bcl-2和bax基因和蛋白的表达分别用MTT法，Real-Time PCR和western blotting法进行检测。此外，进行溴化乙锭/吖啶橙染色以显现凋亡细胞。 4000,3000,2500和2000μg/ ml乳酸链球菌素在mRNA和蛋白质水平上均导致显着的抗增殖影响和增强凋亡指数（bax / bcl-2比率）（p <0.05）。此外，在溴化乙锭/吖啶橙（EB / AO）染色后具有剂量依赖性方式证实凋亡影响。我们的结果表明，乳酸链球菌素可通过内在途径诱导细胞凋亡并导致癌细胞死亡。

Nisin is a polycyclic peptide containing 34 amino acids produced by Lactococcus lactisduring fermentation. Recently, researchers considered nisin as an anticancer peptide. Herein, the authors aim to evaluate the nisin effects on the apoptosis stimulation in the colon cancer cell line. The SW480 cells were exposed to discrepant concentrations of nisin and the cell viability as well as the expression of bcl-2 and bax genes and proteins were surveyed by the MTT assay, Real-Time PCR and western blotting method, respectively. Furthermore, the Ethidium bromide/Acridine orange staining was performed to visualize apoptotic cells. 4000, 3000, 2500 and 2000 μg/ml of nisin led to significant anti-proliferativeimpact and augmentation apoptotic index (bax/bcl-2 ratio) both at mRNA and protein levels (p < 0.05). Furthermore, the apoptotic impacts were demonstrated after Ethidium bromide/Acridine orange (EB/AO) staining to have a dose dependent manner. Our outcome suggested that nisin could induce apoptosis via intrinsic pathways and lead to cancerous cell death.

https://www.sciencedirect.com/science/article/pii/S0882401017308409