摘要

益生菌使用不同的机制帮助维持人类健康。最近，已经公布了几篇涉及这种有益作用的报道，据说这种作用是通过硫醚抗生素的分泌获得的。本研究的重点是从我们当地的利基中分离出的一种益生菌LAB培养物。该LAB已经揭示了水产养殖系统的某些有益效果。对14种有效的胃肠道病原体进行可能的Lantibiotic的存在。对活性进行定性和定量估计，并且用LAB产生的细菌素在不同处理中获得的生长和/或抑制区表示抗微生物活性。对细菌素进行几次温度，pH和不同盐浓度处理，以检查其在这些条件下的稳定性和功效。采用多种方法用于浓缩来自活化的LAB的提取物。使用TLC以及通过吸附吸附方法进行纯化。结果表明细菌素可以被称为可能的硫醚抗生素，其对胃肠道病原体如变形杆菌和副溶血性弧菌非常有效，其已显示被我们局部分离的，产生的和部分纯化的LAB起源的硫醚抗生素抑制。

Probiotics help maintain human health using different mechanisms. Recently, several reports have been published which deal with such beneficial action which is said to be obtained by the secretion of Lantibiotics. This study focuses on one Probiotic LAB culture isolated from our local niche. This LAB has revealed certain beneficial effects in aquaculture systems. Fourteen potent gastrointestinal pathogens were subjected to the presence of the probable Lantibiotic. Both qualitative and quantitative estimation of the activities was carried out and the antimicrobial activity was expressed in terms of either growth obtained and/or zone of inhibition in different treatments with the LAB originated bacteriocin. The bacteriocin was subjected to several temperature, pH and different salt concentration treatments in order to check its stability and efficacy under such conditions. A number of methods were used to concentrate the extract from the activated LAB. Purification was carried out using TLC as well as by adsorptiondesorption method. The results indicate that the bacteriocin can be termed as probable lantibiotic which is quite effective against the gastrointestinal pathogens like Proteus vulgaris and Vibrio parahaemolyticus that have shown to be inhibited by our locally isolated, produced and partially purified LAB originated lantibiotic.

https://www.ijcmas.com/5-3-2016/Arikta%20Biswas%20and%20Rajarshi%20Banerjee.pdf