摘要

本研究通过添加零价铁（ZVI），天然沸石和Dnase研究了粪中性（mAD）和嗜热消化（tAD）猪粪中抗生素抗性基因（ARGs）的命运。随后对微生物群落，intI1，重金属抗性基因（MRGs）和毒力因子（VFs）的变化进行了阐明，以明确影响ARGs减少的因素。结果表明，AD可以实现ARG降低，tAD优于mAD，ZVI和天然沸石可以进一步提高还原性，特别是在mAD中添加天然沸石。添加ZVI和天然沸石后，ARG相对丰度的降低效率分别提高了33.3％和138.5％，但Dnase降低了mAD的ARG降低。除了sulII和tetM之外，大多数ARG可以有效减少。网络分析和部分冗余分析表明，MRG与其后微生物群落的共生对处理中ARGs命运的变化贡献最大。

This study investigated the fate of antibiotic resistance genes (ARGs) during mesophilic (mAD) and thermophilic digestion (tAD) of swine manure through zero valent iron (ZVI), natural zeolite and Dnase addition. Changes of microbial community, intI1, heavy metal resistance genes (MRGs) and virulence factors (VFs) were followed to clarify the influencing factors to ARGs reduction. Results showed that AD could realize ARGs reduction with tAD superior to mAD, and ZVI and natural zeolite could further enhance the reduction, especially for natural zeolite addition at mAD. The reduction efficiency of the relative abundance of ARGs was increased by 33.3% and 138.5% after ZVI and natural zeolite addition, respectively, but Dnase deteriorated ARGs reduction at mAD. Most of ARGs could be reduced effectively except sulII and tetM. Network analysis and partial redundancy analysis indicated that co-occurrence of MRGs followed by microbial community contributed the most to the variation of ARGs fate among treatments.

https://www.sciencedirect.com/science/article/pii/S0960852418303432