摘要

在过去的几十年里，鲍曼不动杆菌因其获取遗传物质并在极端环境中持续存在而成为臭名昭着的医院病原体。最近，人血清白蛋白（HSA）显示出显着增加鲍氏不动杆菌的自然转化频率。该观察结果使我们在HSA诱导下对菌株A118进行转录组学分析以鉴定被HSA改变的基因。我们的结果揭示了296种蛋白质编码基因的统计学显着差异表达，包括与运动性，生物膜形成，代谢，外排泵，胶囊合成和转录调节相关的基因。这些性状的表型分析显示表面相关运动性增加，生物膜形成减少，柠檬酸循环相关酶活性降低，以及与锌可用性相关的存活增加。此外，已知在致病性和抗生素抗性中起作用的基因的表达被改变。这些基因包括与RND型外排泵，VI型分泌系统，铁获得/代谢和β-内酰胺抗性相关的基因。总之，这些结果说明了人类产品，特别是HSA，如何在鲍曼不动杆菌的存活和持久性中发挥重要作用。

In the past few decades Acinetobacter baumannii has emerged as a notorious nosocomial pathogen because of its ability to acquire genetic material and persist in extreme environments. Recently, human serum albumin (HSA) was shown to significantly increase natural transformation frequency in A. baumannii. This observation led us to perform transcriptomic analysis of strain A118 under HSA induction to identify genes that are altered by HSA. Our results revealed the statistically significant differential expression of 296 protein-coding genes, including those associated with motility, biofilm formation, metabolism, efflux pumps, capsule synthesis, and transcriptional regulation. Phenotypic analysis of these traits showed an increase in surface-associated motility, a decrease in biofilm formation, reduced activity of a citric acid cycle associated enzyme, and increased survival associated with zinc availability. Furthermore, the expression of genes known to play a role in pathogenicity and antibiotic resistance were altered. These genes included those associated with RND-type efflux pumps, the type VI secretion system, iron acquisition/metabolism, and ß-lactam resistance. Together, these results illustrate how human products, in particular HSA, may play a significant role in both survival and persistence of A. baumannii.

https://www.ncbi.nlm.nih.gov/pubmed/30282985