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添加黑莓糖浆对尼罗罗非鱼,尼罗罗非鱼对Plesiomonas shigelloides的生长性能,抗氧化和免疫反应以及抗性的影响

发布者:抗性基因网 时间:2018-11-16 浏览量:621

摘要

本研究调查了黑莓糖浆对尼罗罗非鱼(Oreochromis niloticus)生长性能,血液学,非特异性免疫和脾脏基因表达反应的影响。研究中使用了5个平均重量为26.75±2.67g的实验鱼群;其中三个用黑莓糖浆掺入的日粮(7.5gkg-1-BBRY7.5,15gkg-1-BBRY15,30gkg-1- BBRY30)喂养,而无添加剂的基础日粮作为对照。另外,第五组是用氟苯尼考制备的抗生素药物(0.02gkg-1-ABTC)。特别是15gkg-1的膳食黑莓糖浆显着提高了生长性能,呼吸爆发活性,潜在杀伤活性,吞噬活性,吞噬指数,溶菌酶活性,髓过氧化物酶活性,总免疫球蛋白水平,血清SOD活性和血清CAT活性(p <0.05) )。此外,膳食黑莓糖浆增加免疫[热休克蛋白70(HSP70),白细胞介素1,β(IL-1β),肿瘤坏死因子(TNF-α),干扰素γ(IFN-γ),免疫球蛋白M(免疫球蛋白M)的表达水平IgM)]和抗氧化[谷胱甘肽过氧化物酶(GPx)]相关基因在鱼的脾脏中喂养特别是15gkg-1黑莓糖浆(p <0.05)。在20天攻击期结束时,与所有其他治疗组相比,BBRY15和ABTC组的存活率显着更高(p <0.05)。因此,在90天内喂食含有15gkg-1黑莓糖浆的尼罗罗非鱼可能足以改善生长性能,鱼类免疫参数,抗氧化状态以及针对P. shigelloides的存活率,类似抗生素治疗。因此,黑莓糖浆可用作抗生素替代品,用于控制罗非鱼饲料中的P. shigelloides。

The present study investigated the effects of dietary blackberry syrup on growth performance, haematological, non-specific immune and spleen gene expression responses of Nile tilapia, Oreochromis niloticus. Five experimental groups of fish with mean weights of 26.75 ± 2.67 g were used in the study; three of them were fed with blackberry syrup incorporated diets (7.5 g kg-1- BBRY7.5, 15 g kg-1- BBRY15, 30 g kg-1- BBRY30), whereas an additive free basal diet served as the control. Additionally, the fifth group was an antibiotic medicated diet (0.02 g kg-1- ABTC), prepared with the florfenicol. Dietary blackberry syrup especially at 15 g kg-1 significantly increased growth performance, respiratory burst activity, potential killing activity, phagocytic activity, phagocytic index, lysozyme activity, myeloperoxidase activity, total immunoglobulin levels, serum SOD activity and serum CAT activity (p < 0.05). Furthermore, dietary blackberry syrup increased the expression levels of immune [heat shock protein 70 (HSP70), interleukin 1, beta (IL-1β), tumor necrosis factor (TNF-α), interferon gamma (IFN-γ), immunoglobulin M (IgM)] and antioxidant [glutathione peroxidase (GPx)] related genes in the spleen of fish fed with especially 15 g kg-1 blackberry syrup (p < 0.05). At the end of the 20-day challenge period the survival rates were significantly higher in the BBRY15 and ABTC groups compared to all other treatment groups (p < 0.05). As a result, feeding Nile tilapia with a diet containing 15 g kg-1 blackberry syrup over a period of 90 days might be adequate to improve growth performance, fish immune parameters, antioxidant status, as well as survival rate against P. shigelloides, similar to antibiotic treatment. Hence, blackberry syrup can be used as an antibiotics replacer for controlling P. shigelloides in tilapia feed.


https://www.ncbi.nlm.nih.gov/pubmed/30414489