摘要

应用嗜热好氧消化（TAD）进一步降低ARG和重金属抗性基因（HMRG）以及来自厌氧消化（AnD）的污泥中的1类整合子（intI1）。 与AnD不同，在TAD之后没有ARG，HMRG和intI1的富集。 intI1和总ARG的残留基因分数（靶向ARG的总和）分别为0.03和0.08。 两个动力学模型（Collins-Selleck和一阶）描述了靶基因的衰变模式，揭示了TAD期间intI1的快速去除。 在TAD之后，与厌氧消化的污泥相比，人类细菌病原体（HBP）的相对丰度和HBPs物种的数量分别减少至约68％和64％。 因此，在AnD之后的TAD可能具有降低由污水污泥中的ARG，HMRG，intI1和HBP引起的生物风险的高潜力。

Thermophilic aerobic digestion (TAD) was applied to further reduce ARGs and heavy metal resistance genes (HMRGs) as well as class 1 integrons (intI1) in sludge from anaerobic digestion (AnD). Unlike after AnD, there was no enrichment of ARGs, HMRGs and intI1 after TAD. Residual gene fractions of intI1 and total ARGs (sum of targeted ARGs) were 0.03 and 0.08, respectively. Two kinetic models (Collins-Selleck and first-order) described the decay patterns of targeted genes, revealing rapid removal of intI1 during TAD. After TAD, the relative abundance of human bacterial pathogens (HBPs) and the numbers of HBPs species decreased to approximately 68% and 64% compared to anaerobically digested sludge, respectively. Thus, TAD, subsequent to AnD, may possess high potential for reducing biological risks resulting from ARGs, HMRGs, intI1 and HBPs in sewage sludge.

https://www.ncbi.nlm.nih.gov/pubmed/30448677