摘要

我们之前报道了四环素通过其在磷酸钙纳米颗粒（CPNP）中的包埋以及通过纳米抗生素（Tet-CPNP）杀死对四环素（和其他9种抗生素）具有抗性的病原菌志贺氏菌2a的纳米制剂。 ）。在这里，我们报告了Tet-CPNP对由志贺氏菌感染引起的小鼠致命腹泻病“志贺氏菌病”的治疗作用。我们的研究结果表明，Tet-CPNP处理显着降低了由于志贺菌病导致的小鼠胃肠道中大便排泄，结肠长度缩短，体重减轻和细菌定植的发生。组织学和免疫学研究表明，通过Tet-CPNP处理，志贺氏菌感染小鼠肠组织中炎性细胞因子TNF-α，IL-1β和IFN-γ的形态和水平的变化恢复到几乎正常的特征。大量四环素没有抗志贺氏菌作用。因此，四环素的纳米化使旧的，廉价的，广谱的抗生素从过时（由于产生抗性）中恢复活力，使其对于具有有限的医疗预算的腹泻易发生的发展中国家非常有益。

We reported earlier about nano-formulation of tetracycline through its entrapment within calcium-phosphate nano-particle (CPNP) and about killing of pathogenic bacterium Shigella flexnari 2a, resistant to tetracycline (and 9 other antibiotics), by the nanonized antibiotic (Tet-CPNP). Here, we report on therapeutic role of Tet-CPNP against deadly diarrheal disease 'shigellosis' in mice, caused by Shigella infection. Our findings revealed that occurrence of mushy-stool excretion, colon-length shortening, weight-loss and bacterial colonization in gastrointestinal tract of mice due to shigellosis was significantly reduced by Tet-CPNP treatment. Histo- and immuno-logical studies showed that changes in morphology and level of inflammatory cytokines TNF-α, IL-1β and IFN-γ in intestinal tissue of Shigella-infected mice were reverted to almost normal features by Tet-CPNP treatment. Bulk tetracycline had no anti-shigellosis action. Thus, nanonization of tetracycline rejuvenated the old, cheap, broad-spectrum antibiotic from obsolescence (due to resistance generation), making it highly beneficial for diarrhea-prone developing countries with limited health-care budgets.

https://www.ncbi.nlm.nih.gov/pubmed/30448527