摘要

本研究调查了海产品分销渠道中副溶血性弧菌的发生情况，以及致病性和抗生素耐药菌株的发生率。 2017年3月至9月，从韩国的渔业拍卖市场，鱼类市场和在线市场收集了176个样本（126个渔业样本和41个环境样本）。样本富集于多粘菌素培养基，并在CHROM琼脂上铺板弧菌隔离典型的菌落。通过PCR分析这些以鉴定含有hns基因的副溶血性弧菌菌株并检查致病基因（tdh和trh）的存在。筛选分离物的抗生素敏感性（阿莫西林/克拉维酸，氨苄青霉素，氨苄青霉素，氯霉素，氯霉素，环丙沙星，红霉素，庆大霉素和四环素）。 28个样本（16.8％）为副溶血性弧菌阳性，阳性样本中有3个分离株（SMFM201708-OY1，SMFM201709-CO2-2和SMFM201709-CO1-2）致病性，其中含有热稳定直接溶血素（TDH）或热稳定直接相关溶血素（TRH）。这些是与胃肠道症状相关的毒力因子。这三种分离株也对氨苄西林具有抗性。这些结果表明副溶血性弧菌的发病率很高;不到10％。这些是致病性的，并且致病菌株显示出氨苄青霉素抗性。因此，海鲜分销渠道需要食品安全措施。

This study investigated the occurrence of Vibrio parahaemolyticus in seafood distribution channels, and the incidence of pathogenic and antibiotic resistant strains. One hundred sixty seven samples (126 fishery samples and 41 environmental samples) were collected from fishery auction markets, fish markets, and online markets in S. Korea from March to September, 2017. The samples were enriched in polymyxin broth and plated on CHROM agar Vibrio to isolate typical colonies. These were analyzed by PCR to identify V. parahaemolyticus strains containing the hns gene and to examine the presence of pathogenic genes (tdh and trh). The isolates were screened for antibiotic susceptibility (amoxycillin/clavulanic acid, ampicillin, ampicillin sulbactam, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, and tetracycline). Twenty eight samples (16.8%) were V. parahaemolyticus-positive, andfrom the positive samples three isolates (SMFM201708-OY1, SMFM201709-CO2-2 and SMFM201709-CO1-2) were pathogenic which contained thermostable direct hemolysin (TDH) or thermostable direct-related hemolysin (TRH). These are virulence factors associated with gastrointestinal symptoms. These three isolates were also resistant to ampicillin. These results indicate that there is high incidence of V. parahaemolyticus; less than 10%. These were pathogenic, and the pathogenic strains showed ampicillin resistance. Therefore, food safety measures are necessary in a seafood distribution channel. 

https://www.ncbi.nlm.nih.gov/pubmed/30449031