摘要

研究了磺胺嘧啶和环丙沙星对饮用水分配系统（DWDSs）生物膜中微生物的影响。结果证实，16S rRNA对总细菌和细菌属Hyphomicrobium的增加与在磺胺嘧啶和环丙沙星的DWDS中促进抗生素抗性基因（ARGs）和1类整合子（int1）有关。此外，细菌在含有磺胺嘧啶和环丙沙星的DWDS中表现出更高的酶活性，导致细胞外聚合物（EPS）的产生更多。较高含量的EPS蛋白和二级结构β-折叠促进细菌聚集和吸附到管道表面形成生物膜。 EPS可以作为生物膜中微生物的屏障。因此，生物膜细菌群落的变化以及抗生素在DWDSs中16S rRNA对总细菌的增加，这也推动了ARGs的推广。此外，两种抗生素表现出比单独使用磺胺嘧啶和环丙沙星更强的联合作用。

Effects of sulfadiazine and ciprofloxacin on microorganisms in biofilm of drinking water distribution systems (DWDSs) were studied. The results verified that the increases of 16S rRNA for total bacteria and bacterial genus Hyphomicrobium were related to the promotion of antibiotic resistance genes (ARGs) and class 1 integrons (int1) in DWDSs with sulfadiazine and ciprofloxacin. Moreover, the bacteria showed higher enzymatic activities in DWDSs with sulfadiazine and ciprofloxacin, which resulted in more production of extracellular polymeric substances (EPS). The higher contents of EPS proteins and secondary structure β-sheet promoted bacterial aggregation and adsorption onto surface of pipelines to form biofilm. EPS can serve as a barrier for the microorganisms in biofilm. Therefore, the biofilm bacterial communities shifted and the 16S rRNA for total bacteria increased in DWDSs with antibiotics, which also drove the ARGs promotion. Furthermore, the two antibiotics exhibited stronger combined effects than that caused by sulfadiazine and ciprofloxacin alone.

https://www.ncbi.nlm.nih.gov/pubmed/30471500