摘要

衰老是一种不可避免的无处不在的进步，影响着所有生物。共研究了18株乳酸菌（LAB）对腺苷一磷酸激活蛋白激酶（AMPK）的激活作用，AMPK是一种介导寿命延长的细胞内能量传感器。发酵乳杆菌DR9（LF-DR9），副干酪乳杆菌OFS 0291（LP-0291）和瑞士乳杆菌OFS 1515（LH-1515）的无细胞上清液（CFS）显示出最高的AMPK活化并进一步评价。与其他细胞系和对照相比，这三种LAB菌株对AMPK的磷酸化在U2OS和C2C12细胞中更为明显（P <.05）。使用由D-半乳糖（D-gal）诱导的早衰Sprague-Dawley大鼠，给予LAB（10log CFU /大鼠/天）12周，防止D-gal处理的大鼠的端粒长度缩短，与未经处理的对照（P <.05）。与对照相比，LF-DR9降低了腓肠肌和胫骨中p53（一种已知的衰老生物标志物）的基因表达。选择的LAB菌株还增强了大鼠的脂质，肾脏和肝脏特征，表明菌株在预防衰老诱导的代谢疾病方面增加了潜力。菌株LP-0291和LH-1515在胃和肠道模拟条件下显示粘附粘蛋白，无抗生素抗性，耐受和增殖的能力，并抑制病原体大肠杆菌，金黄色葡萄球菌和表皮葡萄球菌的生长，与商业益生菌LF相当-DR9和Lactobacillus sakei Probio 65.本研究深入了解了LAB具有抗衰老作用的潜力，可作为针对抗衰老疗法的新药用食品。

Aging is an inevitable and ubiquitous progress that affects all living organisms. A total of 18 strains of lactic acid bacteria (LAB) were evaluated on the activation of adenosine monophosphate-activated protein kinase (AMPK), an intracellular energy sensor mediating lifespan extension. The cell-free supernatant (CFS) of Lactobacillus fermentum DR9 (LF-DR9), Lactobacillus paracasei OFS 0291 (LP-0291), and Lactobacillus helveticus OFS 1515 (LH-1515) showed the highest activation of AMPK and was further evaluated. The phosphorylation of AMPK by these three LAB strains was more evident in U2OS and C2C12 cells, compared to the other cell lines and control (P < .05). Using premature senescent Sprague-Dawley rats induced by D-galactose (D-gal), the administration of LAB (10 log CFU/rat/day) for 12 weeks prevented the shortening of telomere length in D-gal-treated rats compared to the untreated control (P < .05). LF-DR9 lowered gene expression of p53, a known senescent biomarker, in gastrocnemius muscle and tibia compared to the control. The selected LAB strains also enhanced lipid, renal, and liver profile of rats, suggesting added potential of the strains in preventing aging-induced metabolic diseases. Strain LP-0291 and LH-1515 showed ability to adhere to mucin, no antibiotic resistance, tolerated and proliferated under gastric and intestinal simulated conditions, and inhibited the growth of pathogens Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis, comparable to commercial probiotic LF-DR9 and Lactobacillus sakei Probio 65. This study provided an insight into the potential of LAB for exhibiting antisenescence effects, with potentials as new medicinal foods for targeted antiaging therapies.

https://www.ncbi.nlm.nih.gov/pubmed/30592688