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编码对四环素抗性的基因的分子检测和质粒介导的对印度尼西亚苏卡布米的禽致病性大肠杆菌中喹诺酮的抗性的测定

发布者:抗性基因网 时间:2019-01-03 浏览量:524


目标:
该研究旨在鉴定印度尼西亚Sukabumi的禽大肠杆菌病临床病例中大肠杆菌分离株中编码四环素(TE)抗性和质粒介导的喹诺酮类抗药性的基因。

材料和方法:
2013  -  2017年,印度尼西亚苏卡布米的禽大肠杆菌病临床病例共收集了25个大肠杆菌档案分离株。使用纸盘扩散法测试所有分离物的TE和喹诺酮抗性。通过单聚合酶链式反应(PCR)筛选TE抗性大肠杆菌分离物中tet(A)和tet(B)基因的存在。在喹诺酮抗性大肠杆菌分离株中通过多重PCR检测qnr(A),qnr(B)和qnr(S)基因。

结果:
这项研究的结果表明,25个(76%)大肠杆菌分离株中有19个对土霉素有抗性,64%对TE具有抗性;其中,63.2%和31.5%分别为阳性tet(A)和tet(B)。 25例中有13例(52%)对环丙沙星耐药,36%对恩诺沙星耐诺氟沙星;其中,61.6%为阳性qnr(A),7.7%为阳性qnr(B),23%为阳性qnr(S),7.7%为qnr(A)和qnr(S)阳性。

结论:
该研究表明,大肠杆菌的一些病原体对TE和喹诺酮具有抗性。在印度尼西亚苏卡布米的禽致病性大肠杆菌分离株中引起这种抗性的tet和qnr基因的频率很高。


AIM:
This study aimed to identify genes encoding resistance to tetracycline (TE) and plasmid-mediated resistance to quinolones in Escherichia coli isolates from clinical cases of avian colibacillosis in Sukabumi, Indonesia.

MATERIALS AND METHODS:
A total of 25 E. coli archive isolates were collected in 2013-2017 from clinical cases of avian colibacillosis in Sukabumi, Indonesia. All isolates were tested for TE and quinolone resistance using the disk diffusion method. TE -resistant E. coli isolates were screened for the presence of tet(A) and tet(B) genes by single polymerase chain reaction (PCR). The qnr(A), qnr(B), and qnr(S) genes were detected by multiplex PCR in quinolone-resistant E. coli isolates.

RESULTS:
Result of this study shows that 19 of 25 (76%) E. coli isolates are resistant to oxytetracycline and 64% are resistant to TE; among them, 63.2% and 31.5% were positive tet(A) and tet(B), respectively. 13 out of 25 (52%) are resistant to ciprofloxacin and 36% are resistant to enrofloxacin either norfloxacin; among them, 61.6% were positive qnr(A), 7.7% were positive qnr(B), 23% were positive qnr(S), and 7.7% were positive both of qnr(A) and qnr(S).

CONCLUSION:
This study shows that a few pathogens of E. coli are resistant to TE and quinolone. The frequency of tet and qnr genes that are responsible for this resistance among avian pathogenic E. coli isolates in Sukabumi, Indonesia, was high.

https://www.ncbi.nlm.nih.gov/pubmed/30587892