该研究的目的是通过添加粘土来降低家禽粪便（PM）堆肥中抗生素抗性基因（ARG）的相对丰度，并确定这种效应的机制。根据PM干重，比较了5种粘土添加剂[0％（T1），2％（T2），4％（T3），6％（T4），8％（T5）和10％（T6）]通过添加粘土来探索PM堆肥中ARGs减少的机理。结果证实，在养殖场的初始原始PM中，ARG浓度比国内PM高1.7-3.01倍。高剂量的粘土添加剂在降低ARG中起重要作用，并且是导致治疗组之间ARG丰度显着变化的主要因素。因此，我们建议添加高剂量粘土（HDC）作为降低PM堆肥中ARG最大百分比的有效手段。热图相关性研究证实，在堆肥过程中添加HDC降低了源自鸡饲料的有毒金属的生物可利用部分，并显着影响了ARG分散。

The aim of the study was to reduce relative abundance of antibiotic resistant genes (ARGs) in the poultry manure (PM) compost by adding clay and to determine the mechanism of this effect. Five doses of clay additive [at 0% (T1), 2% (T2), 4% (T3), 6% (T4), 8% (T5) and 10% (T6) based on PM dry weight] were compared to explore the mechanism of reduction in ARGs in the PM compost by the addition of clay. The results confirmed that in the initial raw PM from the breeding farm, the ARG concentrations were 1.7-3.01 times higher than that in the domestic PM. High doses of the clay additive play an important role in reduction in the ARGs and are the main factor responsible for significant variations in ARG abundance between the treatment groups. Therefore, we recommend adding high doses of clay (HDC) as an effective means to reduce the maximum percentage of ARGs in PM compost. A heat map correlation study confirmed that HDC addition during the composting process reduced the bioavailable fractions of toxic metals originating from the chicken feed and significantly impacted ARG dispersion.

https://www.ncbi.nlm.nih.gov/pubmed/30583205