目的：
本研究的目的是确定作为ampC基因携带者的铜绿假单胞菌（铜绿假单胞菌）临床菌株中IMP和OXA基因的存在。

方法：
在该研究中，收集了105个铜绿假单胞菌的临床分离株。使用盘扩散法测定抗生素抗性模式。携带AmpC酶的菌株通过组合圆盘法表征。多重PCR用于鉴定抗性和毒力基因，卡方检验用于确定变量之间的关系。

结果：
在105株铜绿假单胞菌中，对头孢噻肟和氨曲南的抗生素耐药性最高，对大肠杆菌和头孢他啶的耐药性最低。有49株分离株（46.66％）显示出AmpC表型。此外，抗性基因的频率为; OXA48基因85.2％，OXA199,139 3.8％，OXA23 3.8％，OXA2 66.6％，OXA10 3.8％，OXA51 85.2％和OXA58 3.8％。在9株分离株中检测到IMP27基因（8.57％），在11株分离株中检测到IMP3.34（10.47％）。检测到的其他基因包括lasR（17.1％），lasB（18％）和lasA（26.6％）。毒力因子与OX和IMP基因之间存在显着关系（p≤0.05）。

结论：
在该研究中观察到的抗生素抗性和毒力因子之间的关系可能在与铜绿假单胞菌感染相关的暴发中起重要作用。

OBJECTIVES:
The purpose of this study was to determine the presence of IMP and OXA genes in clinical strains of Pseudomonas aeruginosa (P. aeruginosa) that are carriers of the ampC gene.

METHODS:
In this study, 105 clinical isolates of P. aeruginosa were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.

RESULTS:
Among 105 isolates of P. aeruginosa, the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were; OXA48 gene 85.2%, OXA199, 139 3.8%, OXA23 3.8%, OXA2 66.6%, OXA10 3.8%, OXA51 85.2% and OXA58 3.8%. The IMP27 gene was detected in 9 isolates (8.57%) and the IMP3.34 was detected in 11 isolates (10.47%). Other genes detected included; lasR (17.1%), lasB (18%) and lasA (26.6%). There was a significant relationship between virulence factors and the OX and IMP genes (p ≤ 0.05).

CONCLUSION:
The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with P. aeruginosa infections.

https://www.ncbi.nlm.nih.gov/pubmed/30584496