摘要

本研究旨在评估70℃下超嗜热厌氧消化在螺旋霉素发酵残留物预处理中的可行性。通过在5天的固体保留时间内供给市政剩余污泥，超嗜热消化器在3天内从嗜温消化中成功启动，一步升温从35℃升至70℃。 MiSeq测序显示，在温度升高后3天内嗜热发酵细菌群落快速建立，共同菌落，螺旋体藻和铁杆菌的丰度从<0.001％，1.06％和<0.001％增加到33.77％，11.65％和3.42％ ， 分别。在批次实验中评估了用于螺旋霉素残留的超嗜热消化的可行性7天。超嗜热消化显着降低了抗生素浓度，单独的螺旋霉素残留物及其与超嗜热污泥的混合物的去除效率分别为55.3％和99.0％。同时，由于相应宿主的减少，4个大环内酯 - 林可酰胺 - 链霉素抗性基因的丰度也在7天内减少。这些结果表明，嗜热消化很容易从嗜温消化中开始，可能是螺旋霉素残留的合适预处理方法。

This study aimed to evaluate the feasibility of hyperthermophilic anaerobic digestion at 70 °C in the pretreatment of spiramycin fermentation residue. By feeding municipal excess sludge under a solid retention time of 5 days, the hyperthermophilic digester was successfully started up within 3 days from mesophilic digestion by a one-step temperature increase from 35 to 70 °C. MiSeq sequencing showed the fast establishment of thermophilic fermenting bacterial communities in 3 days immediately after the temperature increase, with increases in abundance of Coprothermobacter, Spirochaetaceae_uncultured and Fervidobacterium from <0.001%, 1.06% and <0.001% to 33.77%, 11.65% and 3.42%, respectively. The feasibility of hyperthermophilic digestion for spiramycin residue was evaluated in batch experiments for 7 days. Hyperthermophilic digestion considerably reduced antibiotic concentrations, with removal efficiencies of 55.3% and 99.0% for the spiramycin residue alone and its mixture with hyperthermophilic sludge, respectively. At the same time, the abundances of four macrolide-lincosamide-streptogramin resistance genes were also reduced within 7 days, due to the decrease of their corresponding hosts. These results suggest that hyperthermophilic digestion could easily be started up from mesophilic digestion and might be a suitable pretreatment approach for spiramycin residue.

https://www.ncbi.nlm.nih.gov/pubmed/30566086