摘要

不动杆菌感染的院外流行病学是一个争论的主题。近年来，动物多药耐药性不动杆菌感染的流行程度显着增加。本研究的目的是确定从实验室小鼠中分离的不动杆菌属物种，并测试它们的抗菌药物敏感性。在实验室小鼠的常规微生物监测期间，12个不动杆菌属。被隔离了。通过16S rRNA和rpoB基因测序，7个分离株被鉴定为不动杆菌放射性核素，3个分离株属于不动杆菌基因组14BJ，一个分离物被分类为不动杆菌，一个被分类为不动杆菌属（Acinetobacter sp。）。 ANC 4051.对于所测试的23种抗微生物剂中的21种，最小抑制浓度（MIC）值的分布是均匀的，而对于tulathromycin和链霉素记录了广泛的MIC分布。对于大多数测试的抗生素，记录的MIC值较低。然而，在大多数分离物中记录了非常高的MIC值，其可能使得使用这些物质的治疗方法不成功，用于氟苯尼考，泰妙菌素，替米考星和头孢噻吩。总之，我们记录了具有不同不动杆菌属物种的实验室小鼠的定殖，显示出相似的抗生素易感性特征，可能对不动杆菌流行病学以及定殖动物的饲养和实验有影响。

The extra-hospital epidemiology of Acinetobacter infections is a subject of debate. In recent years, the prevalence of animal multidrug-resistant Acinetobacter infections has increased considerably. The goal of the present study was to specify Acinetobacter species isolated from laboratory mice and to test them for their antimicrobial susceptibility. During routine microbiological monitoring of laboratory mice, 12 Acinetobacter spp. were isolated. By means of 16S rRNA and rpoB gene sequencing, seven of the isolates were identified as Acinetobacter radioresistens, three isolates belonged to Acinetobacter genomospecies 14BJ, one isolate was classified as Acinetobacter pitii and one as Acinetobacter sp. ANC 4051. The distribution of the minimal inhibitory concentration (MIC) values was uniform for 21 of the 23 antimicrobial agents tested, whereas a broad MIC distribution was recorded for tulathromycin and streptomycin. The MIC values recorded were low for the majority of the antibiotics tested. Nevertheless, very high MIC values, which will probably render a therapeutic approach using these substances unsuccessful, were recorded for florfenicol, tiamulin, tilmicosin and cephalothin in most of the isolates. In conclusion, we document colonization of laboratory mice with different Acinetobacter species, displaying similar antibiotic susceptibility profiles, with possible implications in the Acinetobacter epidemiology as well as in the husbandry and experimentation of the colonized animals.

https://www.ncbi.nlm.nih.gov/pubmed/30567464