摘要

环境系统中的移动抗生素抗性基因（ARG）可能对公众健康构成威胁。共存的取代芳香族污染物可以帮助ARG穿过细胞外聚合物质可渗透屏障进入细胞内部，促进ARG传播，但其机制尚不清楚。在这里，我们证明了细胞外基质中移动质粒的特异性抗水解机制对这种促进传播的贡献更大。具体地，用Tb3 +标记的pUC19质粒的荧光微量滴定用于研究与ARG传播相关的取代的芳香质粒复合物的形成。 endA基因和EPS的操作证实这些形成的复合物拮抗EPS介导的质粒水解。傅里叶变换红外光谱（FTIR），X射线光电子能谱（XPS）和计算化学证明，取代基改变芳香分子的极性，使碳在1,3-二氯苯的6位以及不稳定的质子上间苯二胺，苯胺和2-萘酚的（-NH 2 / -OH）主要通过氢键与磷酸酯的去质子化羟基（PO·HC / N / O）相互作用。 ARG传播，结合常数和结合能之间的线性相关性突出了ARG增殖对由D-核糖 - 磷酸酯模板化的功能组合的定量依赖性。

Mobile antibiotic resistance genes (ARGs) in environmental systems may pose a threat to public health. The coexisting substituted aromatic pollutants may help the ARGs cross the extracellular polymeric substance permeable barrier into the interior of cells, facilitating ARG dissemination, but the mechanism is still unknown. Here, we demonstrated that a specific anti-hydrolysis mechanism of mobile plasmid in the extracellular matrix makes a greater contribution to this facilitated dissemination. Specifically, fluorescence microtitration with a Tb3+-labeled pUC19 plasmid was used to study the formation of substituted aromatic-plasmid complexes associated with ARG dissemination. Manipulations of the endA gene and EPS confirmed that these forming complexes antagonize the EPS-mediated hydrolysis of the plasmid. Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and computational chemistry demonstrated that substituents alter the polarity of aromatic molecules, making the carbon at the 6-position of 1, 3-dichlorobenzene as well as the labile protons (-NH2/-OH) of m-phenylenediamine, aniline, and 2-naphthol interact with the deprotonated hydroxy group of the phosphate (P-O···H-C/N/O), mainly via hydrogen bonds. Linear correlations among ARG disseminations, association constants, and bonding energies highlight the quantitative dependency of ARG proliferation on a combination of functionalities templated by D-ribose-phosphate.

https://www.ncbi.nlm.nih.gov/pubmed/30562461