摘要

目前的研究旨在发现活性污泥生物质的动力学和微生物响应，以持续暴露于四环素，四环素是废水中最常检测到的抗生素之一。在实验期关键阶段产生的氧利用率曲线的呼吸计量分析和模型评估表明，除了对生长动力学的温和抑制之外，连续暴露于四环素导致底物储存的完全抑制，并且它与可用的有机物发挥显着的结合作用。碳，导致更少的氧气消耗。此外，内源衰变率增加1.5倍与抗生素抗性基因的存在和产生所决定的维持能量相关，如抗性基因谱所证明的。高通量测序结果表明，持续暴露于四环素导致物种水平的群落结构发生显着变化，因此四环素抗性细菌如Arthrobacter sp和Diaphorobacter sp主导细菌群落。

Current study aimed to discover both kinetic and microbial response of activated sludge biomass to continuous exposure to tetracycline, one of the most frequently detected antibiotics in wastewaters. Respirometric analysis and model evaluation of the oxygen utilization rate profiles generated at critical phases of the experimental period showed that, continuous exposure to tetracycline caused complete suppression of substrate storage aside from mild inhibition on the growth kinetics and it exerted a significant binding action with available organic carbon, leading to less oxygen consumption. Additionally, increase in endogenous decay rates by 1.5 fold was associated with maintenance energy dictated by the presence and production of antibiotic resistance genes, as demonstrated by resistance gene profile. High-throughput sequencing results showed that continuously exposure to tetracycline caused a significant shift in the community structure at species level so that tetracycline resistant bacteria like Arthrobacter sp and Diaphorobacter sp dominated the bacterial community.

https://www.ncbi.nlm.nih.gov/pubmed/30611034