摘要

全基因组测序的扩展和随后抗生素抗性靶标的增加为高通量qPCR（HT-qPCR）铺平了道路，用于在一次运行中分析数百种抗微生物抗性基因（ARG）。对51项选定的研究进行了荟萃分析，以评估过去7年中ARG的丰度趋势。 WaferGenTM SmartChip被发现是用于评估ARG的最广泛使用的HT-qPCR平台。到目前为止，已经在HT-qPCR上分析了来自世界不同地区的约1000个环境样品（不包括生物复制品）。在肠道微生物组研究中报告的计算检测频率和标准化ARGs丰度（ARGs / 16S rRNA基因）显示出与其他环境基质相比ARG低的趋势。人们强调了HT-qPCR数据分析中的差异，这些差异给研究人员在精确解释结果方面造成了困难，并提出了解决这些问题的可能方法。在评论中还讨论了其他扩增技术和用于分析ARG的现场可部署设备的潜力。我们的评论侧重于有关HT-qPCR在抗菌素耐药性领域的作用，现状和未来前景的最新信息。

Expansion in whole genome sequencing and subsequent increase in antibiotic resistance targets have paved the way of high throughput qPCR (HT-qPCR) for analyzing hundreds of antimicrobial resistance genes (ARGs) in a single run. A meta-analysis of 51 selected studies is performed to evaluate ARGs abundance trends over the last 7 years. WaferGenTM SmartChip is found to be the most widely used HT-qPCR platform among others for evaluating ARGs. Up till now around 1000 environmental samples (excluding biological replicates) from different parts of the world have been analyzed on HT-qPCR. Calculated detection frequency and normalized ARGs abundance (ARGs/16S rRNA gene) reported in gut microbiome studies have shown a trend of low ARGs as compared to other environmental matrices. Disparities in the HT-qPCR data analysis which are causing difficulties to researchers in precise interpretation of results have been highlighted and a possible way forward for resolving them is also suggested. The potential of other amplification technologies and point of care or field deployable devices for analyzing ARGs have also been discussed in the review. Our review has focused on updated information regarding the role, current status and future perspectives of HT-qPCR in the field of antimicrobial resistance.

https://www.ncbi.nlm.nih.gov/pubmed/30609875