目的：
本研究的目的是研究从巴西分离的产SPM-1的铜绿假单胞菌ST277的抗性组。

方法：
使用Ion Proton TM系统（Thermo Fisher）进行铜绿假单胞菌HC84的全基因组测序。使用CLC Genomics Workbench 8.0（CLC bio，Aarhus，Denmark）进行从头组装，并使用Prokka管道进行基因预测。

结果与结论：
我们描述了由十三种不同的抗性基因（blaSPM-1，rmtD，aacA4，aadA7，blaOXA-56，blaOXA-396，blaPAO，aph（3'） - 组成的产SPM-1的铜绿假单胞菌ST277（HC84）的抗体组 - IIb，aac（6'）Ib-cr，crpP，catB7，cmx和fosA）。这种特殊的染色体抗性基因包与克隆传播密切相关，并表明该克隆在巴西医院感染中持续存在的重要作用。因此，我们是否可以将“获得性抗性基因的染色体包”视为“ST277内在抗性组”？这是获得性抗性基因，整合和结合元件染色体积累成为成功的细菌病原体的一个例子，并引起人们注意其他物种的进化，这些物种驱动着细菌染色体中多个获得性抗性基因的插入和持续存在。

PURPOSE:
The purpose of this study was to investigate the resistome of a SPM-1-producing P. aeruginosa ST277 isolated from Brazil.

METHODOLOGY:
Whole genome sequencing of P. aeruginosa HC84 was performed using the Ion Proton™ System (Thermo Fisher). De novo assembly was carried out using a CLC Genomics Workbench 8.0 (CLC bio, Aarhus, Denmark) and the gene prediction was performed using the Prokka pipeline.

RESULTS AND CONCLUSION:
We described the resistome of SPM-1-producing P. aeruginosa ST277 (HC84) consisting by thirteen different resistance genes (blaSPM-1, rmtD, aacA4, aadA7, blaOXA-56, blaOXA-396, blaPAO, aph(3')-IIb, aac(6')Ib-cr, crpP, catB7, cmx and fosA). This particular chromosomal pack of resistance genes is strongly associated with clonal dissemination and suggests important role in the persistence of this clone in Brazilian hospital infections. For that reason, could we already consider the "chromosomal pack of acquired resistance genes" like an "ST277 intrinsic resistome"? This is an example of chromosomal accumulation of acquired resistance genes, integrative and conjugative elements into a successful bacterial pathogen and call attention to evolution of other species driving to insertion and persistence of multiple acquired resistance genes in the bacterial chromosome.

https://www.ncbi.nlm.nih.gov/pubmed/30586621