摘要

空肠弯曲杆菌和大肠杆菌是人类胃肠道疾病的主要原因，即使在工业化国家，也会影响公共卫生。本研究旨在评估伊朗阿瓦兹市屠宰的鸡肉、牛肉、羊肉和水牛肉中空肠杆菌和大肠杆菌的发生率和耐药性。共采集380份样本，包括来自工业屠宰场的鸡肉（n=150）、来自传统屠宰场的鸡肉（n=50）、来自当地市场的新鲜包装鸡肉（n=30）和来自阿瓦兹工业屠宰场的牛肉、羊肉和水牛肉（每种肉类50份样本），并测试是否存在弯曲杆菌。SPP。该程序是在42摄氏度微嗜热条件下，一步浓缩普雷斯顿浓缩液，然后在补充血琼脂上电镀24小时。用聚合酶链反应法检测可疑菌落，并用Kirby Bauer圆盘法研究已证实的菌株对各种抗生素的敏感性。F融合方法。总的来说，32个样本（8.40%）被弯曲杆菌污染，羊肉是污染最严重的肉类（24%），而新鲜包装的鸡肉没有受到污染。在32个分离株中，四环素、环丙沙星、氨苄西林和链霉素的耐药率分别为40.60%、34.40%、21.90%和15.60%。此外，还测定了大量耐多抗生素弯曲杆菌。由于动物源性食品是弯曲杆菌感染的最主要来源，因此存在对抗生素有抵抗力的菌株是对公共卫生的潜在风险。

Campylobacter jejuni and C. coli are the main causes of gastrointestinal diseases in humans even in industrialized countries affecting public health. The aim of the current study was to evaluate the occurrence and antibiotic resistance of C. jejuni and C. coli in chicken meat, beef, mutton and water buffalo meat slaughtered in Ahvaz city, Iran. A total of 380 samples including chicken meat from industrial abattoirs (n = 150), chicken meat from traditional abattoirs (n = 50), fresh packed chicken meat from local markets (n = 30) and beef, mutton and water buffalo meat from industrial abattoirs (50 samples for each meat) in Ahvaz,were collected and tested for the presence of Campylobacter spp. The procedure was one-step enrichment in Preston enrichment broth followed by plating on supplemented blood agar for 24 hr under microaerophilic conditions at 42 ˚C. Suspected colonies were tested by polymerase chain reaction assay and susceptibility of the confirmed isolates to various antibiotics was investigated by the Kirby-Bauer disk diffusion method. Overall, 32 samples (8.40%) were contaminated with Campylobacter spp. Mutton was the most contaminated meat (24%), while fresh packed chicken meat were not contaminated. Among the 32 isolates, 40.60%, 34.40%, 21.90%, and 15.60% were resistant to tetracycline, ciprofloxacin, ampicillin, and streptomycin, respectively. Moreover, a high number of multi-antibiotic resistant Campylobacter spp. was determined. Since foods of animal origin are the most sources of Campylobacter infection, the presence of resistant strains to antibiotics is a potential risk to public health.

https://www.ncbi.nlm.nih.gov/pubmed/31183014