摘要

采用了污泥生物干燥的工艺处理残留污水污泥量增加的问题。已经证明，污泥生物干燥有效地降低了抗生素抗性基因（ARG）和移动遗传元件（MGE），而ermF，tetX和sulII响应于微生物群落的动态发展而变得富集。本研究进一步证明，污泥生物干燥产品在当前施用量下的土地利用不会导致土壤中量化ARG的丰度增加，但应注意ARB的持久性。虽然土地应用将ermF，tetX和tetG引入土壤，但很快就会降低到控制水平。此外，土壤类型之间的衰减率不同，红壤是动力学模型中最持久的。 ARG的命运也可归因于土地施用期间微生物群落的动态，并且可降解细胞外DNA的Parasegetibacter属可能在控制ARG中起关键作用。总之，土地施用后的污泥生物干燥可以构成控制ARG传播的有效手段，微生物群落变化对整个处理链中的ARG的命运贡献最大（残留污水污泥→生物干燥过程→土地申请）。

The process of sludge bio-drying has been adopted in response to the increasing amount of residual sewage sludge. It has been demonstrated that sludge bio-drying effectively reduces both antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), whereas ermF, tetX, and sulII become enriched in response to the dynamic development of the microbial community. The present study further demonstrated that the land application of sludge bio-drying products under current application rate did not cause an increase in the abundance of quantified ARGs in the soil but the persistence of ARB should be paid attention. Although land application introduced ermF, tetX, and tetG into the soil, these soon decreased to control levels. Furthermore, the decay rate varied between soil types, with red soil being the most persistent based on kinetics modeling. The fate of ARGs could also be attributed to the dynamics of the microbial community during land application, and the genus Parasegetibacter, which can degrade extracellular DNA, might play a key role in the control of ARGs. In summary, sludge bio-drying following land application could constitute an effective means of controlling the spread of ARGs, and microbial community changes contributed the most to the fate of the ARGs during the entire treatment chain (residual sewage sludge → bio-drying process → land application).

https://www.sciencedirect.com/science/article/pii/S016041201930738X