摘要

公众越来越关注废水处理过程中抗生素抗性基因（ARGs）的命运，它们在处理过程中的持久性及其对接收水体的潜在影响。在这项研究中，我们使用定量PCR（qPCR）来确定来自10个不同欧洲国家的16个废水处理厂（WWTP）废水中9种ARG和1类整合子相关整合酶基因的丰度。为了评估对接收水体的影响，还分析了后者的基因丰度。

在所分析的9个ARG中，有6个在所有废水和河水样本中被检测到。在量化的基因中，inti1和sul1是最丰富的。我们的研究结果表明，欧洲污水处理厂有助于提高受纳水体中的阻力，其具体影响取决于污水量和当地水文条件。研究发现，污水处理厂废水中的args浓度与实施的生物处理步骤的数量成反比，这表明污水处理厂管理的可能选择。

此外，该研究已将blaOXA-58鉴定为可能的抗性基因，用于研究污水处理厂对其接收水的影响的未来研究。

There is increasing public concern regarding the fate of antibiotic resistance genes (ARGs) during wastewater treatment, their persistence during the treatment process and their potential impacts on the receiving water bodies. In this study, we used quantitative PCR (qPCR) to determine the abundance of nine ARGs and a class 1 integron associated integrase gene in 16 wastewater treatment plant (WWTP) effluents from ten different European countries. In order to assess the impact on the receiving water bodies, gene abundances in the latter were also analysed.

Six out of the nine ARGs analysed were detected in all effluent and river water samples. Among the quantified genes, intI1 and sul1 were the most abundant. Our results demonstrate that European WWTP contribute to the enrichment of the resistome in the receiving water bodies with the particular impact being dependent on the effluent load and local hydrological conditions. The ARGs concentrations in WWTP effluents were found to be inversely correlated to the number of implemented biological treatment steps, indicating a possible option for WWTP management.

Furthermore, this study has identified blaOXA-58 as a possible resistance gene for future studies investigating the impact of WWTPs on their receiving water.

https://www.sciencedirect.com/science/article/pii/S0043135419305524