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长期磺胺甲恶唑治疗期间微生物电解池中细菌群落变化和抗生素抗性基因定量

发布者:抗性基因网 时间:2019-10-22 浏览量:1007

摘要

在这项研究中,磺胺甲恶唑用作微生物电解池的电子供体。六个月的运行后,磺胺甲恶唑在三个微生物电解池中的去除效率在3天内分别为77.60%,87.55%和92.53%,与初始添加浓度成正比。然而,具有开路和无微生物的微生物电解池中,去除效率分别仅为51%和8%。更高的磺胺甲恶唑浓度和持续的电刺激引起更快的生物电化学反应,从而增强了磺胺甲恶唑的降解。细菌群落分析显示,主要的功能菌群——Proteobacteria和Synergistetes,随着抗生素浓度的增加而增殖。 qPCR结果表明,微生物电解细胞生物膜和流出物中抗生素抗性基因和整合素的拷贝数明显低于传统生物处理系统。因此,在微生物电解池中,抗生素抗性基因的产生和传播可能是减少的挑战。


In this study, sulfamethoxazole served as the electron donor for microbial electrolysis cells. After 6 months of operation, the removal efficiencies of sulfamethoxazole in three microbial electrolysis cells were 77.60%, 87.55%, and 92.53% for a 3-day period and were directly proportional to the initial added concentrations. However, the removal efficiencies in the microbial electrolysis cells with open circuits and without microorganisms were only 51% and 8%, respectively. Higher sulfamethoxazole concentrations and sustained electrical stimulation caused faster bioelectrochemical reactions, thereby enhancing sulfamethoxazole degradation. Bacterial community analysis revealed that Proteobacteria and Synergistetes, which are the main functional phyla, proliferated with increased antibiotic concentrations. The qPCR results indicated that the copy numbers of antibiotic resistance genes and integrons in microbial electrolysis cell biofilms and effluents were distinctly lower than those in traditional biological treatment systems. Thus, the generation and dissemination of antibiotic resistance genes might be a diminished challenge in microbial electrolysis cells.


https://www.sciencedirect.com/science/article/pii/S0960852419314002