摘要

我们分析了两个不同地理区域的埃塞俄比亚土著鸡盲肠微生物群的全基因组：Afar（AF）区（Dulecha，平均海拔730 abovem）和Amhara（AM）区（Menz Gera Midir，3300 m）。通过宏基因组学分析，我们发现微生物种群主要由拟杆菌和硬菌纲组成。我们在两组之间鉴定了2210个共有基因。 LEfSe表明，与AM相比，AF中的原杆菌，地杆菌，cronobacter， Alloprevotella 和Dysgonomonas的分布明显丰富。使用KEGG，eggNOG和CAZy数据库进行的分析表明，代谢，遗传信息处理，环境信息处理和细胞过程的途径显着丰富。发现功能丰富与土著鸡的营养吸收和微生物定位有关。我们还研究了ARG，发现AF中的LSM，头孢菌素和四环素等抗生素比AM明显丰富。

We analyzed the whole genomes of cecum microbiomes of Ethiopian indigenous chickens from two distinct geographical zones: Afar (AF) district (Dulecha, 730 m above mean sea level) and Amhara (AM) district (Menz Gera Midir, 3300 m). Through metagenomic analysis we found that microbial populations were mainly dominated by Bacteroidetes and Firmicutes. We identified 2210 common genes between the two groups. LEfSe showed that the distribution of Coprobacter, Geobacter, Cronobacter, Alloprevotella, and Dysgonomonas were significantly abundant in AF than AM. Analyses using KEGG, eggNOG, and CAZy databases indicated that the pathways of metabolism, genetic information processing, environmental information processing, and cellular process are significantly enriched. Functional abundance was found to be associated with the nutrient absorption and microbial localization of the indigenous chickens. We also investigated the ARGs and found antibiotics like LSM, cephalosporin, and tetracycline were significantly more abundant in AF than AM.

https://www.ncbi.nlm.nih.gov/pubmed/31759120