摘要

     在这里，我们发现光催化（PC）可以引起细菌的应激反应，这种应激反应可以暂时介导一些机制，如外排泵、生物膜的形成、突变率的增加（3-5倍的变化），从而提高抗生素耐受性。不同抗生素灭活99%的细菌所需的时间不同，多粘菌素（PB）为30~150 min，四环素（TET）为55~135 min，环丙沙星（CIP）为14~40 min，链霉素（SM）为22~35 min，阿奇霉素（azithromycin）为29~45 min。之后，随着抗生素的选择，具有较高抗生素耐受性的细菌可以更快地进化具有抗生素耐药性。耐药菌对PB、TET、CIP、SM、AZI的耐药倍数分别为32、32、16、4，均高于正常菌。我们的结果表明，除了抗生素外，PC等不利的环境因素可能有助于细菌耐药性的形成。

     Here, we found that the photocatalysis (PC) could elicit bacterial stress response that transiently mediate some mechanism such as efflux pumps, biofilm formation, increased mutation rates (3–5-fold changes), leading to the improved antibiotic-tolerance. The time needed to inactivate 99 % of bacteria increased from 30 to 150 min for polymyxin (PB), 55 to 135 min for tetracycline (TET), 14–40 min for ciprofloxacin (CIP), 22–35 min for streptomycin (SM), and 29–45 min for azithromycin (AZI). Afterwards, the bacteria with higher antibiotic-tolerance could evolve to antibiotic-resistance faster with subsequent antibiotic selection. The fold change of antibiotic-resistance level was up to 32 for PB, 32 for TET, 16 for CIP, 16 for SM and 4 for AZI, which are higher than those of normal bacteria. Our results suggest that, besides antibiotic, unfavorable environmental factors such as PC might give assistance to development of bacterial antibiotic-resistance potentially.

     https://www.sciencedirect.com/science/article/abs/pii/S0926337320302447