摘要

堆肥并不能完全有效地降低动物粪便中的抗生素抗性基因（ARGs）。以菜花和玉米秸秆为膨化剂，研究了不同处理条件对猪粪中ARGs去向的影响。结果表明，与对照组相比，添加微生物制剂或玉米秸秆与菜花比例（1:12）可显著降低大部分ARG的绝对丰度（平均480倍）。主成成分分析表明，细菌群落与ARGs的丰度显著相关，说明微生物群落对堆肥过程中ARGs的变化有影响。冗余度和网络分析证实了个别ARGs（qnrS、blaAmpC、blaTEM-1）的变化模式受宿主菌（假单胞菌、克雷伯杆菌和嗜盐菌）的选择性和环境变量（TN、NH3-N、TOC和pH）的影响。这些发现有助于优化堆肥条件，从而降低ARGs传播的风险。

Composting is not completely effective in reducing antibiotic resistance genes (ARGs) in animal manure. This work studied the effects of different treatment conditions on the fate of ARGs in composting swine manure with cauliflower and corn straw as bulking agents. The results showed that the addition of microbial agents or the ratio of corn stalks to cauliflower (1:12) could significantly decrease the absolute abundances of most ARGs (an average of 480 times) compared with the control treatment. Principal component analysis indicated that bacterial communities were significantly correlated with ARG abundance, suggesting that microbial communities have an impact on ARG variation during co-composting. Redundancy and Network analysis confirmed the changing patterns of individual ARGs (qnrS, bla_AmpC, bla_TEM-1) were influenced by the selectivity of host bacteria (Pseudomonas, Klebsiella, and Halocella) and environmental variables (TN, NH₃-N, TOC, and pH). These findings helped to optimize composting conditions, thereby reducing the risk of ARGs spread.

https://www.sciencedirect.com/science/article/abs/pii/S096085241931898X