摘要

本文研究了一个大型淡水池塘养殖系统水体和沉积物中的抗药性基因（ARGs）谱。采用qPCR阵列定量分析了16S、Tetx、Tetw、TetG、Intll和Sull等ARGs，并通过16srrna基因测序分析了微生物群落结构。检测到大量ARGs（288）。沉积物中ARG含量显著高于水体，平均多检测到15个基因（p<0.01）。沉积物样品显示出更高的分类多样性和更高的γ变形菌门、β-变形菌们和黄杆菌的丰度。抗生素抗性基因与繁殖周期有显著的相关性。池塘样品的分类学多样性显著高于沟渠样品（p<0.05），说明池塘养殖系统可以作为一个局部蓄水池，将ARGs扩散到农村社区的水环境中。

This study examined the profiles of antibiotic resistance genes (ARGs) in water and sediments from one large-scale freshwater pond farming system. A qPCR array was used to quantify ARGs (16S, Tetx, Tetw, TetG, Intll, and Sull) and microbial community structure was analyzed by 16S rRNA gene sequencing. A large number of ARGs (2 8 8) were detected. The ARG richness of the sediments was significantly higher than that of water and an average of 15 more genes were detected (p < 0.01). Sediment samples showed significantly higher taxonomic diversity and higher abundance of Gammaproteobacteria, Betaproteobacteria, and Flavobacteria. A significant correlation was observed between antibiotic resistance genes and breeding periods. The taxonomic diversity of the samples in ponds was significantly higher than that in ditch samples (p < 0.05), suggesting that pond farming systems could act as a local reservoir to spread ARGs into aquatic environments of rural communities.

https://www.sciencedirect.com/science/article/pii/S0048969719346170